人巨细胞病毒UL135基因在临床低传代分离株中的多态性研究  

Polymorphism of human cytomegalovirus UL135 gene in low passage clinical isolates

作　　者：孙峥嵘[1] 阮强[1] 何蓉[1] 马艳萍[1] 齐莹[1] 吉耀华[1] 

机构地区：[1]中国医科大学附属第二医院病毒研究室,辽宁沈阳110004

出　　处：《中国医科大学学报》2006年第1期17-19,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30170986)

摘　　要：目的：研究人巨细胞病毒（HCMV）UL135基因编码序列在临床低传代分离株中的多态性，探讨HCMV基因多态性与其感染引起不同临床疾病之间的关系。方法：对29株经荧光定量PCR方法检测HCMV．DNA为阳性的临床低传代分离株的细胞培养上清液进行HCMVUL135全序列PCR扩增，并对PCR扩增阳性的25例标本进行序列测定及分析。结果：29株临床低传代分离株有25株PCR扩增阳性。25株分离株UL135开放读码框架（0RF）长度均与Toledo株相同，其核苷酸的变异率为0．1％-2．6％。与Toledo株相比，部分分离株具有氨基酸翻译后修饰位点的新增或缺失。25株临床分离株UL135蛋白质二级结构预测结果显示了第28位、313—317位、161—163位、171位氨基酸均有蛋白质结构二级的改变。结论：25株HCMV临床低传代分离株UL135基因及其编码产物的氨基酸序列比较保守，但仍存在一定程度的多态性，未发现UL135基因的变化与HCMV先天感染导致的不同疾病存在着相关联系。Objective： To investigate the polymorphism of human eytomegalovirus （HCMV） UL135 gene in the low passage clinical isolates and to study the relationship between the polymorphism and diseases caused by congenital HCMV infection. Methods： The entire HCMV UL135 gene regions of 29 clinical isolates in which positive HCMV-DNA was detected by using fluorescence quantitative pelymerase chain reaction （FQ-PCR） were amplified by polymerase chain reaction （PCR）. Amplified PCR products were sequenced, and the sequence were analyzed. Results： Of 29 isolates, 25 were amplified succesofuUy. The length of UL135 open reading frame （ORF） in these 25 clinical isolates was similar to that of Toledo sequence. The nucleotide mutation rate of UL135 protein was 0. 1% to 2.6%. Additional or deleted sites of posttranslational modification of UL135 protein were found in all clinical isolates. Condusion： All DNA and deduced amino acid sequences of UL135 gene shared great similarity among HCMV clinical isolates regardless of their pelymorphism. No correlation was found between the pelymorphism of UL135 gene and the diseases caused by congenital HCMV infection.

关 键 词：巨细胞病毒 UL135基因 基因多态性 

分 类 号：R373[医药卫生—病原生物学]