冷血浆停搏液对离体幼兔心脏冠脉流出液CPK、LDH、ATP影响的研究  

Effects of cold plasma cardioplegia on lactate dehydrogenase,creatine phosphokinase,and adenosine triphosphate in coronary outflow of isolated immature rabbit heart

作　　者：吴秀英[1] 龙波[1] 李树森[2] 崔健君[1] 

机构地区：[1]中国医科大学附属第二医院麻醉科,辽宁沈阳110004 [2]中国医科大学附属第二医院心脏外科,辽宁沈阳110004

出　　处：《中国医科大学学报》2006年第2期142-143,共2页Journal of China Medical University

基　　金：辽宁省科学技术计划基金资助项目(619041)

摘　　要：目的:观察冷血浆停搏液对离体幼兔心脏冠脉流出液肌酸磷酸激酶(CPK)、乳酸脱氢酶(LDH)、三磷酸腺苷(ATP)的影响。方法:选择新西兰幼兔28只,离体心脏复跳后灌注不同的停搏液。对照组灌注托马斯(Thom as)停搏液,实验组灌注冷血浆停搏液(血浆与Thom as停搏液按4∶1比例配制)。停跳30 m in重新复跳后,测定冠脉流出液LDH、CPK及ATP含量。结果:实验组冠脉流出液LDH、CPK及ATP含量均明显低于对照组。结论:冷血浆停搏液可使未成熟心肌的心肌酶和ATP漏出减少,对未成熟心肌可能有保护作用。Objective： To investigate the effects of cold plasma cardioplegia on lactate dehydrogenase （LDH）, creatine phosphokinase （CPK）, and adenosine triphosphate （ATP） in the coronary outflow of isolated immature rabbit heart. Methods： The hearts of 28 New Zealand white rabbits were isolated and peffused with cold （ 10℃ ） St. Thomas cardioplegic solution in group C （n = 14） or mixture of cold eardioplegic solution and plasma （ St. Thomas solution： plasma = 1 ： 4） in group P （ n = 14）. The contents of LDH, CPK, and ATP were measured when the hearts rebeated 30 minutes after cardiac arrest. Results： The contents of LDH, CPK, and ATP in the coronary outflow in group P were significantly lower than those in group C. Conclusion： Cold plasma eardioplegia may reduce the leakage of myocardium enzyme and ATP in immature myocardium and thus protect the immature myeocardium in rabbits.

关 键 词：冷血浆停搏液 冠脉流出液 

分 类 号：R54[医药卫生—心血管疾病]