秋水仙素诱导兰考泡桐同源四倍体  

AUTOTETRAPLOID INDUCTION OF Paulownia elongata WITH COLCHIONE

作　　者：范国强[1] 杨志清[1] 曹艳春[1] 刘飞[1] 贾峰[1] 

机构地区：[1]河南农业大学泡桐研究所,河南郑州450002

出　　处：《核农学报》2006年第6期473-476,547,共5页Journal of Nuclear Agricultural Sciences

基　　金：河南省高校创新人才基金资助项目(2002012)

摘　　要：在含不同浓度秋水仙素的MS+0.1mg/L NAA+15mg/L BA(最适器官发生)双层培养基上进行兰考泡桐四倍体植株诱导试验,并通过变异植株根尖细胞染色体观察和叶片单细胞DNA含量测定进行倍性分析。结果表明,预培养12d的兰考泡桐叶片在秋水仙素浓度为10mg/L的双层培养基上预培养处理24h时,四倍体诱导率最高达到23.4%。变异植株叶片比二倍体大,叶片长宽比变小,叶片单个气孔变大,气孔密度变小。Autotetraploids from diploidy leaves of Paulownia elongata on the double layer MS media supplemented with 0.1 mg/L NAA and 15 mg/L BA （optimal organogenetic medium to the leaves of diploidy seedlings） with different colchicines concentrations were induced, chromosome numbering of the plant root tip ceils and DNA content analysis of the leaf single cell were used to analyse the autotetra ploids. The results indicated that the highest tetraploid induction rate could reach 23.4% when the leaves precuhured on the optimal organogenetic MS medium for 12d and treated with 10 mg/L colchione on the double layer media for 24h in vitro. Compared with diploidy seedlings of P. elongate, leaf length and stomata size of tetraploid becomes bigger, the leaf length/width ratio and stomata density becomes smaller.

关 键 词：泡桐 四倍体 秋水仙素 诱导 

分 类 号：S792.43[农业科学—林木遗传育种]