水稻空间诱变的遗传变异及突变体的AFLP分子标记  

GENETIC VARIATION OF SPACE FLIGHT CARRIED RICE AND MUTANT ANALYSIS BY AFLP MOLECULAR MARKER

作　　者：蒲志刚[1] 张志勇[1] 郑家奎[2] 向跃武[1] 张志雄[1] 蔡平钟[1] 文春描[1] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066 [2]四川省农业科学院水稻高粱研究所,四川泸州646000

出　　处：《核农学报》2006年第6期486-489,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家"863"计划(2002AA2070002;2001AA2410112);四川省财政厅高新技术研究专项和四川省应用基础研究项目资助(04JY029-004-3);四川省农业科学院青年基金

摘　　要：搭载“神舟三号”航天飞船的水稻纯合种子在返回地面后SP3代种子出现颖壳、茎杆和叶片的主脉变异为金黄色的突变体,其他性状无明显变异,经遗传分析,颜色变异属一对隐性基因控制的性状变异。同时利用AFLP分子标记的方法,对航天诱变的水稻突变体进行基因组对比分析,通过聚丙烯酰胺电泳寻找突变体(黄金1号)多态性DNA片段,经过一系列引物筛选,找到了5条多态性DNA条带,对开展突变体基因功能研究和杂交水稻聚合育种有十分重要的意义。Rice seeds were carried by ＂Shenzhou No. 3＂ space shuttle, a mutant with golden chaff, stem and leaf was selected and named Golden 1 after the seeds returned to the earth. Except the golden color, other traits of Golden 1 are no obviously different with its original material H9808. Genetic analysis identified that color variation was control by a pair of recessive gene. The DNA fragments of the mutant were compared with its parent by AFLP molecular markers. Five specific bands were found through a serial selections.

关 键 词：空间诱变 突变体 颜色 AFLP 分子标记 

分 类 号：S511[农业科学—作物学]