约氏疟原虫感染大鼠肝脏、腹腔巨噬细胞B7·1、B7·2、TGF-β、IFN-γ基因表达研究  

STUDY ON EXPRESSION OF B7.1,B7.2,IFN-γ,TGF-β GENES OF RAT INFECTED WITH PLASMODIUM YOELII

作　　者：宋蓓[1] 张锡林[1] 段建华[1] 黄复生[1] 

机构地区：[1]第三军医大学病原生物学教研室,重庆400038

出　　处：《寄生虫与医学昆虫学报》2006年第4期193-198,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金资助项目(30371261)

摘　　要：建立约氏疟原虫——斯氏按蚊——哺乳动物模型，在感染约氏疟原虫后2h、12h、24h、48h、72h分别提取大鼠肝脏和腹腔巨噬细胞总RNA，通过RT-PCR定量分析发现在宿主体感染约氏疟原虫后2～72h时间段内，B7．1表达水平没有变化，而B7．2、TGF-β、IFN-γ转录水平显著上调。该现象提示疟原虫子孢子入侵引起宿主体内免疫相关基因B7．1、B7．2、TGF-β、IFN-γ mRNA转录水平发生改变，可能与哺乳动物机体针对疟原虫感染产生的免疫防御、免疫调控密切相关。We established an animal model of Plasmodium yoelii-Anopheles stephensi-mammal for the following experiments. In 2 h, 12 h, 24 h, 48 h, 72 h, the total RNA of liver and macrophagus from rat infected by Plasmodium yoelii sporozoites were extracted. Then we quantitatively analyzed the expression of B7.1, B7.2, IFN-γ, TGF-β mRNA by RT-PCR. The result indicated that the transcription of B7.2, IFN-γ, TGF-β of rat infected with Plasmodium yoelii sporozoites were up-regulated, while B7.1 remained steadily. The changes of B7.2, TGF-β, IFN-γ mRNA expression suggested these genes were likely involved in the mammal host immune system and related regulations after the rat was infected by Plasmodium yoelii sporozoites.

关 键 词：B7.1 B7.2 约氏疟原虫 APCs 转录水平 

分 类 号：R383[医药卫生—医学寄生虫学]