丹参粉针剂对自发性高血压大鼠心肌细胞凋亡的影响  

Effect of salvia miltiorrhiza on cardiomyocyte apoptosis in spontaneously hypertensive rats

作　　者：杨海波[1] 邓杰[1] 王明明[1] 郑智[2] 

机构地区：[1]浙江省舟山市人民医院重症治疗室 [2]华中科技大学同济医学院附属同济医院急诊科

出　　处：《中国临床药理学与治疗学》2006年第10期1115-1118,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省中医药管理局青年基金资助项目(№2004Y033)

摘　　要：目的探讨丹参粉针剂对自发性高血压大鼠(SHR)心肌细胞凋亡的作用。方法18只引种繁殖的雄性SHR随机分成3组,每组6只对照组育至第8周处死;丹参组从第8周给药,丹参粉针剂每天1 g·kg-1腹腔注射,第18周处死;高血压组用相同容量蒸馏水替代丹参针腹腔注射,余同丹参组。测量大鼠尾动脉收缩压(SBP)及左心室重量指数(LV-MI) ,采用TdT介导的原位末端缺口标记法(TUNEL)检测心肌细胞凋亡,用S-P免疫组化法检测心肌细胞Bcl-2基因蛋白表达。结果与对照组比较,高血压组SBP、LVMI、心肌细胞凋亡指数(AI)显著增加,Bcl-2蛋白阳性表达指数(PEI)降低;与高血压组比较,丹参组LVMI、AI显著降低,PEI增高,差异均有统计学意义。结论长期应用丹参粉针剂治疗,能显著上调SHR心肌细胞Bcl-2基因蛋白表达,抑制心肌细胞凋亡,预防高血压左室肥厚的形成。AIM： To observe the effect of salvia miltiorrhiza on cardiomyocyte apoptosis in spontaneously hypertensive rats （SHR）. METHODS： 18 male SHR were divided into 3 groups at random （ n = 6, each） ： the base group, in which rats were killed in the 8th week; the salvia group, in which salvia miltiorrhiza （ 1 g·kg^-1·d^-1） was injected intraperitoneally from the 8th week to the 18th week; the control group, in which the same volume of vehicle was injected in the same manner. Systolic blood pressure （SBP） and left ventricular mass index （LVMI） were measured. The TUNEL method was employed to evaluate cardiomyocyte apoptosis, and the immunohistochemistry method was applied to detect the protein expression of Bcl-2. RESULTS： Compared with the base group, the SBP, LVMI and apoptosis index increased markedly in control group （ P 〈 0.05）. Simultaneously, the positive expression index of Bcl-2 protein decreased significantly in control group （ P 〈 0.05）. Treated with salvia miltiorrhiza, the LVMI and cardiomyocyte apoptosis decreased. Simultaneously, the positive expression index of Bcl-2 protein increased, whereas SBP changed slightly but significantly （ P 〈 0.05 ）. CONCLUSION： The study shows that chronic use of salvia miltiorrhiza can upgrade the protein expression of Bcl-2 gene, decrease the cardiomyocyte apoptosis and inhibit the development of left ventricular hypertrophy in spontaneously hypertensive rats.

关 键 词：丹参 高血压 大鼠 细胞凋亡 心肌 BCL-2 蛋白 

分 类 号：R965.2[医药卫生—药理学]