烧伤延迟复苏时大鼠心肌细胞线粒体呼吸功能的变化  

Cbanges of mitochondria function of myocardial cell in burnt rats after delayed fluid resuscitation

作　　者：李锐[1] 邱振中[1] 

机构地区：[1]中国人民解放军第三医院烧伤科,陕西宝鸡721004

出　　处：《中国烧伤创疡杂志》2006年第4期250-252,共3页The Chinese Journal of Burns Wounds & Surface Ulcers

基　　金：兰州军区2005年度医药卫生科研课题(编号LXH-2005003)

摘　　要：目的:观察严重烧伤延迟复苏时大鼠心肌细胞线粒体呼吸功能的变化。方法:88只大鼠随机分为正常对照组(A组)、即时复苏组(B组)和延迟复苏组(C组),B组和C组造成20％总体表面积Ⅲ度烧伤,并测定伤后1h,3h,6h,24h,48h线粒体呼吸Ⅲ、Ⅳ态及呼吸控制率(RCR);测定线粒体呼吸链NADH-CoQ还原酶、琥珀酸脱氢酶活力。结果:①烧伤后B、C组心肌细胞线粒体RCR和NADH-CoQ还原酶显著低于A组(P<0．05);C组显著低于B组(P<0．05);②B组心肌细胞线粒体RCR和NADH-CoQ还原酶活力复苏3小时代偿性增高,但后期进一步降低;③伤后3小时内,B、C组心肌细胞线粒体内琥珀酸脱氢酶活力无显著降低(P >0．05);复苏后期显著低于A组(P<0．05),C组显著低于B组(P<0．05)。结论:严重烧伤大鼠延迟复苏时会加重心肌细胞线粒体呼吸功能损伤。Objective： To investigate the changes of mitoehondria respiratory function of myocardial cell in burnt rats after delayed fluid resuscitation. Method： 88 rats were randomly divided into normal control group （A）, immediate fluid resuscitation group （B） and delayed fluid resuscitation group （ C ）. Rats in groups A and B were subjected to full - thickness thermal injury with an area of 20% TBSA. Their mitoehondria respiration state III and IV, respiratory control rate （ RCR）, and the activity of NADH - CoQ reductase and succinate dehydrogenase of mitoehondria respiratory chain were determined at 1 h, 3 h, 6 h, 24 h and 48 h post injury. Result： 1. After burn, the RCR and the activity of NADH - CoQ reductase in groups B and C were much lower than that in group A （ P 〈 0. 05）, and in group C , NADH - CoQ reductase activity was obviously lower than that in group B （ P 〈 0. 05 ）. 2. The RCR and the activity of NADH - CoQ reductase in groups B had a compensatory increase in 3 h after resuscitation, but decreased again later. 3. In 3 h after burn, the activity of succinate dehydrogenase in groups B and C did not change obviously （ P 〉 0. 05 ）, but it was much lower than that in group A in the later stage of resuscitation （ P 〈 0.05 ）, and in group C, succinate dehydrogenase activity was obviously lower than that in group B （ P 〈 0. 05 ）. Conclusion： Delayed fluid resuscitation results in more severe injury of mitoehondria respiratory function of myocardial cell in burnt rats.

关 键 词：烧伤 延迟复苏 心肌细胞 线粒体 

分 类 号：R644[医药卫生—外科学] Q244[医药卫生—临床医学]