汉滩病毒体外诱导热休克蛋白70的表达  

Quantitative analysis of the patterns of heat shock protein 70 expression induced by Hantaan virus infection in vitro

作　　者：余璐[1] 马恒[2] 段春光[1] 孙玉静[1] 杨守京[1] 

机构地区：[1]第四军医大学西京医院病理科,基础部病理学教研室,西安710032 [2]第四军医大学西京医院生理学教研室,西安710032

出　　处：《中华传染病杂志》2006年第6期378-381,共4页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目(30271186)

摘　　要：目的定量研究汉滩病毒(HTNV)体外诱导细胞热休克蛋白70(HSP70)及HSP70 mRNA的表达规律。方法以HTNV敏感细胞株Vero-E6为对象,体外实验性感染Hantaan 76-118。采用原位杂交、免疫细胞化学染色法及逆转录聚合酶链反应(RT-PCR)观察感染后72 h内HSP70的表达并加以定量分析。结果与模拟感染组相比,Hantaan 76-118感染Vero-E6细胞后0．5 h．HSP70 mRNA开始升高,于感染后12 h达高峰,并持续高表达至72 h(P＜0．05)。Hantaan 76-118感染后2 h可检测到HSP70蛋白表达增加,于感染后12 h达高峰,并持续高表达至72 h(P＜0．05)。结论体外感染HTNV可诱导Vero-E6细胞产生热休克反应并表达HSP70,这种快速、持久的应激反应,可能对HSP70持续发挥其细胞保护作用具有重要意义。Objective To explore the pattern and quantify the heat shock protein （HSP）70 and HSP70 mRNA in Vero-E6 cells after infection with Hantann virus （HTNV）. Methods The expression of HSPT0 and change of its mRNA level were detected by immunocytochemical staining, nucleic acid hybridization in situ and RT-PCR. Results In situ hybridization and RT-PCR were used to evaluate the level, of HSP70 mRNA during Hantaan 76-118 infection. HSP70 mRNA increased 0.5 h after infection, reached its peak by 12 h and gradually declined to steady state level by 72 h（vs. sham infected group, P 〈 0.05）. The expression of HSP70 protein induced by Hantaan 76-118 infection was evaluated by quantitative immunocytochemical staining. HSP70 increased 0.5 h after infection, reached its peak by 12 h and decreased at 72 h after infection （vs. sham infected group, P 〈 0.05）. Conclusions HSP70 can be induced directly by HTNV infection at both mRNA and protein levels. It provides a basis for the further study of the pathogenesis, prevention and treatment of hemorrhagic fever with renal syndrome（HFRS）.

关 键 词：汉滩病毒 热休克蛋白质70 热休克反应 

分 类 号：R373[医药卫生—病原生物学]