Effects of ginkgo biloba extract on the expressions of IL-1β,TNF-α,IL-10 and IL-10R in heart of atherosclerotic rats  

作　　者：焦亚斌 芮耀诚 李铁军 杨鹏远 邱彦 

机构地区：[1]Department of Pharmacology School of Pharmacy,Second Military Medical University

出　　处：《Journal of Medical Colleges of PLA(China)》2006年第6期347-351,共5页中国人民解放军军医大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No. 30271509)

摘　　要：Objective： To study whether the anti-AS effect of ginkgo biloba extract （GbE） was related with inhibitory effects on the expression of IL-1β, TNF-α and up-regulation of IL-10 and IL-10R in the heart of atherosclerotic （AS） rats. Methods： The experimental model of AS rats were established by intraperitioneal injection of vitamin D3 with high fat and cholesterol diet. All rats were divided into 3 groups： control, AS and GbE. GbE （100 mg/kg） was administered to rats by ig. After 8 weeks, the expression of IL-1β, TNF-α, IL-10, and IL-10R in the heart of AS rats were detected by enzyme-linked immunosorbant assay, reverse transcriptase polymerasechain reaction and Western blotting. Results： The protein and mRNA expressions of IL-1β, TNF-α, IL-10 and IL-10R were markedly higher in AS group than those in control group （P〈0.01）. The protein and mRNA expressions of IL-1β and TNF-α were markedly lower in GbE group than those in AS group; while the protein and mRNA expressions of IL-10 and IL-10R were markedly higher in GbE group than those in AS group （P〈0.01）. Conclusion： GbE has significant inhibitory effects on proinflammatory cytokine IL-1β, TNF-α. The up-regulation of anti-inflammatory cytokine IL-10, IL-10R that may he partially responsible for its anti-AS effects.Objective: To study whether the anti-AS effect of ginkgo biloba extract (GbE) was related with inhibitory effects on the expression of IL-1β, TNF-αand up-regulation of IL-10 and IL-10R in the heart of atherosclerotic (AS) rats. Methods : The experimental model of AS rats were established by in-traperitioneal injection of vitamin D3 with high fat and cholesterol diet. All rats were divided into 3 groups: control,AS and GbE. GbE (100 mg/kg) was administered to rats by ig. After 8 weeks, the expression of IL-1β, TNF-α, IL-10, and IL-10R in the heart of AS rats were detected by enzyme-linked immunosorbant assay, reverse transcriptase polymerasechain reaction and Western blotting. Results:The protein and mR-NA expressions of IL-1β,TNF-α,IL-10 and IL-10R were markedly higher in AS group than those in control group (P<0. 01). The protein and mRNA expressions of IL-1βand TNF-αwere markedly lower in GbE group than those in AS group; while the protein and mRNA expressions of IL-10 and IL-10R were markedly higher in GbE group than those in AS group (P<0. 01). Conclusion: GbE has significant inhibitory effects on proinflammatory cytokine IL-1β, TNF-α. The up-regulation of anti-inflammatory cy-tokine IL-10,IL-10R that may be partially responsible for its anti-AS effects.

关 键 词：atherosclerosis cytokine INTERLEUKIN-1 tumor necrosis factor INTERLEUKIN-10 Ginkgo biloba RATS 

分 类 号：R543[医药卫生—心血管疾病]