“益肾清火”方对大鼠牙周炎症和免疫功能的影响  

Effect of Yishenqinghuo recipe on periodontal inflammation and immunity of rats with experimental periodontitis

作　　者：罗礼君[1] 束蓉[1] 宋忠臣[1] 吴安平[1] 俞瑾[2] 史桂英[3] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔内科,上海市口腔医学研究所,上海200011 [2]复旦大学附属妇产科医院中西医结合科,上海200011 [3]上海交通大学医学院细胞生物学教研室,上海200025

出　　处：《上海口腔医学》2006年第6期605-609,共5页Shanghai Journal of Stomatology

基　　金：国家"十五"科技攻关计划(2004BA720A26);上海市自然科学基金(03ZR14081)

摘　　要：目的观察中药“益肾清火”方对大鼠牙周炎症和免疫功能的影响。方法选择12个月龄SD大鼠,分为A、B、C、D共4组。A组为空白对照组,B组为牙周炎造模组,C组为造模后灌服中药高剂量组,D组为造模后灌服中药等效剂量组。牙周炎造模成功后,A、B组动物灌喂生理盐水,C、D组动物灌喂中药3个月。分别采用脱钙骨切片、流式细胞术及ELISA法测定用药前后动物牙周组织、外周血中T细胞的分类及比值、IL-2和IL-1β水平的变化,并采用SAS6.04软件包对数据进行单因素方差分析。结果牙周炎大鼠灌服“益肾清火”方3个月后,与B组相比,C、D组的牙周组织炎症明显改善;各组外周血中CD4+T/CD8+T的比值分别为3.55±0.94、2.42±0.75、3.23±1.14和3.29±0.83,B组比其余3组低(P<0.05);IL-2与IL-1β分别为(36.03±2.63/179.04±17.29)pg/ml、(25.18±3.08/306.09±13.38)pg/ml、(38.44±2.58/176.33±45.38)pg/ml和(36.81±2.45/182.13±43.97)pg/ml。与其余3组相比,B组IL-2水平显著下降,IL-1β显著升高(P<0.01),但以上指标在两用药组之间无显著差别(P>0.05)。结论中药“益肾清火”方能改善实验性牙周炎动物的牙周炎症,对动物机体免疫有一定的调节作用。PURPOSE： To evaluate the effect of Yishenqinghuo recipe on periodontal inflammation and immunity of rats with experimental periodontitis. METHODS： 12 months old Spague-Dawley rats were used in this study. 78 rats were randomly divided into 4 groups, group A： control group （with no periodontitis, fed with the same dosage of saline as group D）; group B： model group （with periodontitis, fed with the same dosage of saline as group D）; group C： high dosage group （with periodontitis, fed with double dosage of medicine as group D）; group D： equivalent dosage group （with periodontitis, fed with clinical equivalent effective dosage of medicine）. After being garaged with medicine/saline for 3 months, the periodontium was analyzed through histological slices; and the amount of CD4^＋T, CD8^＋T, the ratio of CD4^＋T/CD8^＋T, IL-2 and IL-1β in peripheral blood were detected by flow cytometry and ELISA. All the results were analyzed by ANOVA, with the use of SAS 6.04 software package. RESULTS： It was found that the periodontal inflammation of group C and D were improved significantly; the ratio of CD4^＋T/CD8^＋T in peripheral blood for this 4 groups were 3.55±0.94, 2.42±0.75, 3.23±1.14 and 3.29±0.83; the level of IL-2 and IL-1β were （36.03±2.63/179.04±17.29）pg/ml, （25.18±3.08/306.09±13.38）pg/ml, （38.44±2.58/176.33±45.38）pg/ml and （36.81±2.45/182.13±43.97）pg/ml. Compared with group B, the ratio of CD4^＋T/CD8^＋T for group C and D was significantly rised （P〈0.05）; the level of IL-2 increased significantly and IL-1β decreased accordingly （P〈0.01）. However, there was no significant difference between group C and D （P〉0.05）. CONCLUSION： From this study, we conclude that Yishenqinghuo recipe can improve the periodontal inflammation and adjust the immunity of rats with experimental periodontitis. Supported by National ＂Tenth Five-year＂ Key Science and Technology Project （Grant No.2004BA720A26） and Natural Science Foundation of Shanghai

关 键 词：牙周炎 中医药 流式细胞术 细胞因子 

分 类 号：R285[医药卫生—中药学]