小鼠胚胎干细胞向成牙本质样细胞的诱导分化  

Induction of mouse embryonic stem cells forming odontoblast-like cells by co-culture with pulp fibroblast

作　　者：江中明[1] 季佩红 刘军[1] 唐月军[1] 李生娇[1] 李文林[3] 

机构地区：[1]第二军医大学长海医院口腔科,上海200433 [2]上海市杨浦区牙防所,上海200082 [3]第二军医大学细胞生物教研室,上海200433

出　　处：《上海口腔医学》2006年第6期653-656,共4页Shanghai Journal of Stomatology

基　　金：上海市科技发展基金(03ZR14027)

摘　　要：目的探讨胚胎干细胞(ES细胞)向成牙本质样细胞诱导分化的方法。方法首先通过悬浮培养的方式,将ES细胞诱导分化形成拟胚体,然后将拟胚体细胞与牙髓成纤维细胞通过Transwell培养系统共培养,研究拟胚体细胞向成牙本质样细胞的分化情况。结果RT-PCR结果显示,拟胚体细胞与牙髓成纤维细胞共培养10d后,可检测到成牙本质细胞的特征性分子——牙本质涎磷蛋白(DSPP)的表达;共培养15d后,表达有所增强。而单独培养的拟胚体细胞,则始终未检测到DSPP的表达。结论通过与牙髓成纤维细胞共培养,可以促进胚胎干细胞向成牙本质样细胞分化。PURPOSE： To investigate the methods of induction of mouse embryonic stem cells to differentiate into odontoblast-like cells by co-culture with pulp fibroblast. METHODS： By suspension culture, embryonic stem cells were induced to form embryoid bodies. Then the cells from embryoid bodies were co-cultured with pulp fibroblast in Transwell system for differentiation toward odontoblast-like cells. RESULTS： By RT-PCR analysis, embryoid body cells gave rise to the cell population expressing DSPP, a specific odontoblast cell marker, after 10 days of co-culture with pulp fibroblast and the expression of DSPP was enhanced after 15 days of co-culture. On the other hand, the expression of DSPP was not detected in the isolately cultured embryoid body cells. CONCLUSIONS： Embryonic stem cells can be induced into odontoblast-like cells by co-culture with pulp fibroblast. Supported by Science and Technology Development Fund of Shanghai Municipality （Grant No. 03ZR14027）.

关 键 词：胚胎干细胞 成牙本质样细胞 分化 共培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]