锁阳多糖提取工艺的研究  

Study on Extraction Process for Polysaccharide of Cynomorium songaricum Rupr.

作　　者：刘晔玮[1] 邸多隆[2] 葛斌[3] 刘丽华[1] 张永利[1] 

机构地区：[1]兰州大学公共卫生学院 [2]中国科学院兰州化学物理研究所甘肃省天然药物重点实验室 [3]甘肃省人民医院药剂科,兰州730000

出　　处：《天然产物研究与开发》2006年第6期1007-1009,共3页Natural Product Research and Development

基　　金：甘肃省中医药科研课题(GZK-2005-015)

摘　　要：以浸膏得率和浸膏中多糖质量分数综合评价,利用L9(34)正交实验从水提和醇沉两方面优选锁阳中水溶性多糖最佳提取工艺条件,为锁阳活性多糖的开发提供科学依据和实验基础。最佳水提工艺条件为:m(锁阳):v(水)=1:12,回流提取0.5 h,提取2次;最佳醇沉工艺条件为:在m(生药):v(水提液)=1 g:2 mL溶液中加入乙醇,使φ(乙醇)=70%,静置6 h。With the extraction rote and the mass fraction of polysaccharide in extract as the assessment index,the optimum extracting conditions for polysaccharide of Cynomorium songaricum Rupr. were investigated by the orthogonal design. The optimum water extraction condition was refluxing for twice （0.5 h/times） with m（ Cybinirugn sngarrium Popr. ） ：v （H2O） = 1 ： 12; the optimum alcohol sedimentation condition was adding ethanol till φ（ CH3CH2OH） = 70% in the water extraction solution of 0.5 g/mL Cynomorium songaricum Rupr. ,depositing for 6 h.This extraction processs is available for industrial production and can provide the basis of the experimemt to Cynomorium songaricum Rupr.＇s developmemt.

关 键 词：锁阳 提取工艺 正交实验 多糖质量分数 浸膏得率 

分 类 号：R284.2[医药卫生—中药学] Q949.762.9[医药卫生—中医学]