金定鸭的核型、C-带和Ag-NORs研究  

Studies on the Karyotype, C-band and Ag-NORs of Jingding Ducks

作　　者：赵捷[1] 李慧芳[2] 朱云芬[2] 赵旭庭[3] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院家禽研究所,江苏扬州225003 [3]江苏畜牧兽医职业技术学院,江苏泰州225300

出　　处：《中国家禽》2006年第24期99-102,共4页China Poultry

基　　金：国家高技术研究发展计划项目(863-2001AA243082);国家自然科学基金资助项目(30170673)

摘　　要：采用外周血淋巴细胞培养-空气干燥法制备染色体标本,对金定鸭染色体核型和带型进行了研究。核型研究结果表明:金定鸭体细胞染色体数目2n=78,染色体基本臂数为NF=84(♂)或NF=83(♀),1号染色体为亚中央着丝粒染色体,2号染色体为中央着丝粒染色体,Z染色体具有明显短臂,属亚端着丝粒染色体,其余常染色体和W性染色体均为端着丝粒染色体;性染色体按大小顺序排列,Z为4号,W为7～8号。C-带研究发现:W染色体整条深染,极易识别。Ag-NORs研究结果表明:Ag-NORs常位于1号、2号、3号、6号染色体及Z染色体和一对小染色体上,Ag-NORs数目分布范围为1～6,平均每个细胞的Ag-NORs数雌鸭中为3.04雄鸭中为3.06。This experiment studied the chromosome slides of Jinding ducks by the peripheral blood lymphocyte culture-drying method,and analyzed the Jinding ducks＇ karyotype and band patterns. The result of karyotype analysis showed that the diploid chromosome number of Jingding duck was 2n=78,the total number of chromosome arms were NF=84 （♂）or NF=83 （♀）,and chromosome 1 was submetacentric,chwmosome 2 was metaeentrlcs,chromosome Z was a subtelocentric chromosome,with an obviously short ann,chromosome W and the other autosomes were telcentries, According to the measured relative length,chromosome Z was chromosome 4,chromosome W was ranged between chromosome 7 and chromosome 8. Study on Jingding ducks＇ C-bands showed that chromosome W was easily indentified and totally dark-stained. The result of Ag-NORs showed that Ag-NORs were located in chromosome 1,2,3,6, sexchromosome Z and a pair of mierochromosomes,and the Ag-NORs number varied from 1 to 6,average Ag-NOlts number of each cell was 3.04 in females and 3.06 in males.

关 键 词：金定鸭 核型 C-带 AG-NORS 

分 类 号：S834[农业科学—畜牧学]