猪2型圆环病毒ORF2基因片段的克隆与序列分析  

Cloning and Sequence Analysis of ORF2 Gene of Porcine Circovirus Type 2

作　　者：曹三杰[1] 肖驰[1] 文心田[1] 马晓平[1] 

机构地区：[1]四川农业大学动物科技学院动物传染病与基因芯片实验室

出　　处：《四川农业大学学报》2006年第4期435-438,共4页Journal of Sichuan Agricultural University

基　　金：四川省重点科技攻关项目(01NG018-01);四川农业大学青年创新基金

摘　　要：利用ArrayDesigner2.0软件设计PCV2引物直接从PDNS病料肺组织中PCR扩增获得PCVCQB7基因片段,用pMD18-T载体连接、克隆获得PCVCQB7重组质粒。序列测定和与标准毒株对位比对分析PCVCQB7核苷酸序列全长624bp,系PCV2ORF2基因片段,与PCV2分离株的核苷酸序列相似性在80%以上,与PCV1在66%以下,采用最大似然法构建的系统发生树分析发现PCVCQB7位于PCV2主枝上,并与国内和欧洲分离株Imp.1147、304、375、QD、GD-TS、SH、24657_NL毒株亲缘关系较近形成一小分枝,表明PCVCQB7为引起PDNS的PCV2扩增产物。PCVCQB7 was amplified from lung samples porcine dermatitis and nephmpathy syndrome （PDNS） directly by PCR and sequeneed. It is located in ORF2 of PCV type 2 （PCV2） and is 624 bp length. By comparison of PCVCQB7 and PCV, its nueleotides identities are above 80 % （PCV2） and below 66% （PCV1）, while its nueleotides and amino aeids identities are above 94% and 90% with isolates from PDNS. Phylogenetie tree based on PCVCQB7 nueleotide sequenee by Maximum likelihood shows that the PCVCQB7 is located in PCV2 major braneh and forms a minor branch with Imp. 1147, 304,375, 24657-NL, QD, GD - TS, SH isolates from European and China. It is coneluded that PCVCQb7 is ORF2 segment of PCV2 caused PDNS.

关 键 词：猪2型圆环病毒 基因克隆 序列分析 

分 类 号：S858.285.3[农业科学—临床兽医学] S852.65[农业科学—兽医学]