龙胆草新品系组培快繁的研究  

A Study on Rapid Breeding of a New System of Gentiana scabra Bunge Via Tissue Culture

作　　者：李昌禹[1] 孔祥义[1] 王英平[1] 卢淑波[1] 艾军[1] 王志清[1] 赵宏辉 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109 [2]吉林市船营区越北镇政府,吉林吉林132011

出　　处：《特产研究》2006年第4期37-38,共2页Special Wild Economic Animal and Plant Research

摘　　要：目的:通过组织培养方法快速繁育龙胆草新品种。方法:以龙胆草幼嫩的茎为外植体,培养在MS培养基上,通过胚状体途径获得龙胆草组培苗芽丛。结果:在MS+BA0.5mg/L+NAA0.2mg/L的培养基上获得了组培苗,在1/2MS+NAA1mg/L的培养基上获得了生根的组培苗,移栽到田园土中获得了完整的龙胆草小植株。本研究初步建立了龙胆草快速育种模式。Objective：To generate the new system Gentiana scabra Bunge via tissue culture. Method：Tender stems of Gentiana scabra Bunge were cultured as explant in MS medium and clump of buds were acquired via embryoid. Result： Tissue culture plants were acquired in MS medium （BA0.5mg/L＋NAA0.2 mg/L） and root in 1/2MS medium （NAAlmg/L）; plantlets survived when transferred in plates with garden soil, We built a method of rapid breeding of Gentiana scabra Bunge in this study initially.

关 键 词：龙胆草 新品系 组培 育种 

分 类 号：S567.23[农业科学—中草药栽培] Q943.1[农业科学—作物学]