筛选放线菌活性产物防治烟草黑胫病的初步研究  

Screening of the active metabolites of actinomycetes for control tobaccoblack shank

作　　者：曾莉[1] 朱孟沼 刘树芳[1] 李一青 杨佩文[1] 李铭刚 尚惠[1] 崔晓龙 李家瑞[1] 文孟良 

机构地区：[1]云南省农业科学院农业环境资源研究所,云南昆明650205 [2]云南省生物资源保护与利用重点实验室,云南昆明650091

出　　处：《西南农业学报》2006年第6期1074-1077,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金重点项目(2004c0002z)资助

摘　　要：研究结果表明，通过菌丝培养后并以生长速率法测定抑菌活性，对225份放线菌发酵液提取物进行室内毒力测定，筛选到对烟草黑胫病菌Phytophthora nicotianae菌丝生长抑制率在70．00％-94．60％的有28份，占总发酵液提取物的12．44％；对菌丝生长抑制率70％以上的发酵液提取物进行盆栽烟苗防治效果测定，防治效果在40％以上的有19份；并对防治效果筛选模型方法进行了试验比较。Through the method of mycelia growth velocity determination,the existing 225 fermentation broth extracts of actinomycetes were selected for in vitro toxicological testing. The research results indicated that 28 samples screened ,which occupied the 12.44 % of the total fermentation broth extractions samples, showed the mycelia growth inhibition rate at 70.00 % - 94.60 %. The samples with the mycelia grmcth inhibition rate over 70 % were selected for further prevention effect testing in cultivated tobacco and 19 samples exhibited the prevention effect rate over 40 %. Different prevention effect modeling methods were also compared in this paper.

关 键 词：放线菌 发酵液提取物 烟草黑胫病 筛选 防治效果 

分 类 号：S572[农业科学—烟草工业]