马铃薯A病毒云南分离物外壳蛋白基因的克隆与序列分析  

Cloning and sequencing of the coat protein gene of Potato virus A from Yunnan

作　　者：丁铭[1] 方琦[1] 张丽珍[1] 李婷婷[1] 董家红[1] 张仲凯[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南昆明650223

出　　处：《西南农业学报》2006年第6期1078-1081,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金重点项目资助(2005C0012Z)

摘　　要：根据马铃薯病毒A(Potato virusA,PVA)外壳蛋白(CP)基因序列设计合成的一对引物,以带病毒植株总RNA为模板,RT-PCR扩增得到长约800 bp的目的片段。将目的片段克隆至pGEM-T Easy载体并进行了序列测定,测得全长为807 bp的PVA CP基因。测序结果与PVA其他分离物CP基因序列比较,其氨基酸同源性最高可达98.5%。根据GenBank中PVA CP氨基酸序列建立了病毒的系统进化树并对PVA不同分离物CP氨基酸序列差异性进行了分析。Using specific primers which were designed based on Potato virus A （PVA） coat protein （CP） gene sequences,a DNA fragment （800bp） was amplified by reverse transcription polymerase chain reaction （RT-PCR） using the total RNA extracted from the potato plant showed mosaic symptom. The fragment was cloned into pGEM-T Easy vector and then sequenced. Sequence comparison showed it had high sequence homology with CP gene of other PVA isolates with 98.7% at nucleic acid level and 98.5 % at amino acid level. Phylogenetic tree based on CP amino acid sequences of PVA was established and the relationship among the isolates was compared.

关 键 词：马玲薯 A病毒 云南分离物 外壳蛋白基因 序列分析 

分 类 号：S532[农业科学—作物学]