反义抑制MnSOD转基因拟南芥构建及其抑制效果的分析  

Transgenic Arabidopsis Down-regulated the Expression of MnSOD by Anti-sense DNA

作　　者：王义华[1] 徐梅珍[1] 曾黎明[1] 陈蓉蓉[1] 蒋伦伟[1] 甘义忠 吴小飞 

机构地区：[1]江西农业大学理学院,南昌330045 [2]玉山县能源建设委员会,玉山334700 [3]江西邓家埠水稻原种场,余江335200

出　　处：《江西农业大学学报》2006年第6期915-918,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金(30660087);江西省自然科学基金(0630034);江西省科技厅"工业攻关"项目(赣科发计字[2006]138号)

摘　　要：为阐明植物的线粒体MnSOD在逆境适应性反应中的作用,本研究利用分子生物学技术,以拟南芥Mn-SOD基因上游序列构建其反义序列表达载体,转化拟南芥获得转基因植株。经Northern b lot鉴定发现转基因拟南芥MnSOD mRNA水平降低,NBT法检测转基因拟南芥MnSOD活性下降,表明该反义DNA序列对MnSOD基因表达具有明显的抑制效果。In order to reveal the roles of MnSOD in response to stresses in plant, the transgenic Arabidopsis expressing the anti - sense DNA of up - stream sequence of MnSOD in Arabidopsis was constructed. Northern blot analysis showed that the expression levels of MnSOD in transgenic plants were lower than those in wild type plants, and the activities of SOD were decreased in transgenic Arabidopsis. The results indicated that the anti - sense DNA sequence in transgenic Arabidopsis inhibited the expression of MnSOD successfully. This study provides fundamental model plant for further investigation for the functions of MnSOD in plants under stresses.

关 键 词：MNSOD 反义抑制 拟南芥 逆境 

分 类 号：Q753[生物学—分子生物学]