微核监测技术在蚕豆种质资源耐盐性评价中的应用研究  

Application of micronucleus test technology to salt-tolerance evaluation in broadbean gepmplasm resources

作　　者：郑开斌[1] 李爱萍[1] 臧春荣[2] 纪荣昌[1] 

机构地区：[1]福建省农业科学院作物研究所,福建福州350013 [2]福建省农业科学院科研处,福建福州350003

出　　处：《福建农业学报》2006年第4期366-369,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金资助项目(B0310021)

摘　　要：研究了100份蚕豆种质的根尖细胞微核率及盐敏指数差异情况，以盐胁迫发芽试验的相对盐害率为参照指标，分析微核率、盐敏指数和相对盐害率间的相关关系。结果表明：微核监测技术适用于蚕豆种质的耐盐性鉴定，供试的100份蚕豆种质在3．0％NaCl盐溶液胁迫下的微核率变异幅度为8．3‰～15．3‰．盐敏指数变异幅度为0．33～2．56；以盐敏指数为耐盐指标，对上述100份蚕豆种质资源进行耐盐力分级，其中Ⅰ级占3％、Ⅱ级占9％、Ⅲ级占18％、Ⅳ级占32％、Ⅴ级占38％。The difference of root-tip cell micronucleus frequency and salt-sensitive index among 100 Vicia faba varieties were studied. Correlativity among micronucleus frequency, salt-sensitive index and relatively salt damage rate were analyzed by the relatively damage rate of seedlings under salt-stress. Mieronueleus test technology was adapted to appraise salt-tolerance of Vicia faba, the Vicia faba micronucleus frequency were 8. 3‰ to 15.3‰, the saltsensitive index were 0.33 to 2.56 under 3.0%NaC1. Salt-tolerance of 100 Vacia faba varieties was divided into 5 grades by salt-sensitive index, among whith, 3 in Ⅰ , 9 in Ⅱ, 18 in Ⅲ, 32 in Ⅳ and 38 in Ⅴ.

关 键 词：微核监测 微核率 耐盐性 盐胁迫 盐敏指数 种质资源 蚕豆 

分 类 号：S643.6[农业科学—蔬菜学]