制备式等电聚焦鉴定湖南产五步蛇蛇毒磷脂酶A_2的等电点及纯度  

ISOELECTRIC POINT AND PURITY OF PHOSPHOLIPASE A 2 FROM Agkistrodon Acutus VENOM IDENTIFIED BY PREPARATIVE ISOEIECTRIC FOCUSING

作　　者：范礼斌[1,2] 冉永禄[1,2] 

机构地区：[1]安徽医科大学生物教研室 [2]中国科学院昆明动物研究所

出　　处：《蛇志》1996年第4期3-4,共2页Journal of Snake

摘　　要：制备式等电聚焦测得湖南产五步蛇蛇毒磷脂酶Ａ２为单一的吸收峰（ＯＤ２８０），等电点为５．３２。Preparative isoelectric focusing detected that the isoelectric point of phospholipase A 2 from Agkistrodon acutus venom,which is single peak of protein absorbance (OD 280 ),is 5 32,which confirmed the previously purified enzyme was a purity

关 键 词：五步蛇 蛇毒 蛇类 磷脂酶A2 制备式等电聚焦 

分 类 号：Q959.620.6[生物学—动物学]