鸡新城疫病毒的分离与鉴定  

Isolation and Identification of Avian NDV

作　　者：刘栋[1] 廉中华 崔言顺[1] 李建亮[1] 朱玉成 

机构地区：[1]济南市畜牧局,山东济南250002 [2]平阴县孝直畜牧兽医站,山东平阴250400 [3]平阴县畜牧局,山东平阴250400

出　　处：《家畜生态学报》2006年第6期120-123,共4页Journal of Domestic Animal Ecology

基　　金：山东省自然科学基金项目(编号:Y2002D20;2002-2005)

摘　　要：从山东某鸡场的产蛋下降而无其他典型新城疫(Newcastle Disease)症状的高抗体蛋鸡群中分离到一株病毒。经过试验鉴定,该分离株具有血凝性,且可被ND标准阳性血清所抑制和中和,不能被AI(H5亚型与H9亚型)标准阳性血清抑制;该病毒的最小致死量病毒致死鸡胚的平均时间(h)为50.4 h,1日龄SPF鸡脑内接种分离病毒致病指数(ICPI)为1.85,6周龄SPF鸡静脉接种致病指数(IVPI)为2.42,回归试验鸡出现了新城疫症状和病变,经外源病毒检测及回归试验该病毒株被确定为新城疫病毒,并属强毒型,命名为ShD-5-04。One virus isolated from Shandong current outbreak ND chicken farms that only showed egg dropping and without any typical symptom was studied. The results of idenfication showed that the ioslation is able to agglutinate chicken red blood cells, which can be inhibited and neutralized by avian Newcastle disease virus （NDV） standard antiserum ,but can＇t be inhibited by avian influenza virus （AIV） standard antiserum （H5 and H9 subtype） and EDS-76 standard antiserum. Its MDT,ICPI and IVPI are 50.4,1.85 and 2.42 respectively. The ND pathological changes and symptoms also appeared in the challenged chickens. The results disclosed that the isolation is virulent strain of Newcastle disease virus. The isolation was named as ShD-5-04.

关 键 词：新城疫病毒 分离 鉴定 

分 类 号：S811.5[农业科学—畜牧学]