通过16S rDNA全序列分析及鉴定牛沙门氏菌  

Dentification of Salmonella Typhimurium by 16S rDNA Sequence Analysis

作　　者：马广强[1] 杨少华[2] 王宏梅[1] 李建斌[1] 张维军[1] 栾婧婧[1] 林树乾[1] 高运东[2] 赵宏坤[2] 仲跻峰[2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东省农业科学院奶牛研究中心,山东济南250100

出　　处：《家畜生态学报》2006年第6期137-140,共4页Journal of Domestic Animal Ecology

基　　金：国家"十五"科技攻关奶业专项(2002BA518A16);山东省"三0"工程项目资助;项目编号:2003-3009

摘　　要：通过16S rDNA全序列的DNA同源性分析以及PCR-RFLP分析，对从山东某奶牛场送检的腹泻死亡的犊牛内脏中分离到的一株沙门氏菌进行了血清型鉴定，得到其系统发育树状图。系统发育树分析表明，该沙门氏菌菌株与GENEBANK上发表的27株沙门氏菌的16S rDNA同源性较高，为97-99％，该沙门氏菌菌株与鼠伤寒沙门氏菌LT2（Salmonella typhimurium LT2）的同源性最高，达到了99．7％，表明该沙门氏菌菌株为鼠伤寒沙门氏菌。A pathogenic bacterial strain S1, isolated from bowels of diarrhea calves in Shandong provice in 2004,was identified as typical Salmonella by Biological Characterization. Comparative 16S rDNA sequence analysis revealed that strain S1 was most related to the genus of Salmonella. The homology between strain S1 and Salmonella was 97% to 99%. Phylogentic analysis showed that strain S1 exhibited the highest level of homology to Salmonella typhimurium LT2（99.6%）. By 16S rDNA PCR-RFLP analysis,it is confirmed that strain S1 was Salmonella typhimurium.

关 键 词：犊牛 鼠伤寒沙门氏菌 16S RDNA 系统发育学分析 

分 类 号：S811.5[农业科学—畜牧学]