金黄色葡萄球菌荚膜多糖的制备及生物学特性  

Preparation and Biological Characteristics of Staphylococcus Aureus Capsular Polysaccharide

作　　者：林树乾[1] 杨少华[1] 张维军[2] 王洪梅[1] 高运东[1] 李国升 李庆剧 赵宏坤[1] 仲跻峰[1] 

机构地区：[1]山东省农业科学院奶牛中心,山东济南250100 [2]山东农业大学动物科技学院,山东泰安271018 [3]山东佳宝乳业工程研究院,山东济南250100

出　　处：《家畜生态学报》2006年第6期153-155,共3页Journal of Domestic Animal Ecology

基　　金：国家"乳业"重大科技专项(2002BA518A16);山东三0工程项目(2003-3009)

摘　　要：采用Cetavlon、冷酚、不同浓度乙醇沉淀、离心等步骤精制的荚膜多糖,用生物学、免疫学等方法分析检测其理化特性及免疫原性。结果表明:提取的荚膜多糖具有良好的多糖特性、结构特征和免疫原性。Purified Staphylococcus aureus capsular polysaccharide was prepared by a process consisting of extraction with Cetavlon and cold phenol,precipitation by ethanol, centrifugation, and so on, then analyzed by biological and immunological methods, detect the physicochemical characteristic and immunogenicity of it. Results shows the prepared staphylococcus aureus capsular polysaccharide showed good biological and structural characteristics as well as immunogenicity.

关 键 词：金葡菌 荚膜多糖 生物学特性 

分 类 号：S811.5[农业科学—畜牧学]