考马司亮蓝G-250染色法测定羊毛酶减量  

Measuring weight loss of the wool treated by enzyme with Coomassie Brilliant Blue

作　　者：张茜[1] 张健飞[1] 

机构地区：[1]天津工业大学,天津300160

出　　处：《毛纺科技》2006年第12期39-42,共4页Wool Textile Journal

摘　　要：文章研究了考马司亮蓝G-250染色法测定羊毛酶减量的可行性,分析了羊毛织物经过木瓜蛋白酶s、avinase、woolase 3种酶单独处理、经过3种不同氧化前处理后,再经过同种酶处理和不同种酶处理的织物减量率与工作残液中析出的蛋白质浓度的线性关系。实验表明,不同的处理工艺不会影响这种线性相关性,同时也充分证明了考马司亮蓝G-250染色法测定羊毛酶减量的稳定性。This paper studied the feasibility of measuring weight loss of the wool which treated by enzyme with Coomassie Brilliant Blue,analyzed the linearity relationship of the weight less of the wool fabrics and protein concentration separated out in spent baths .The wool fabrics was treated by three different kinds of enzymes：pawpaw protease,savinase,woolase alone and by three different kinds of pretreatment followed by the same kinds of enzyme or the different kinds of enzymes.The research proved that the linearity relationship was not related to the technics and the stability of the weight loss measured by Coomassie Brilliant Blue. This kind of method was not only propitious to the research of the wool shinkproofing treatment using enzyme in the lab, hut also made an important role if enzyme used in the practical production.

关 键 词：羊毛 考马司亮蓝G-250 酶 减量 

分 类 号：TS193[轻工技术与工程—纺织化学与染整工程]