蒙自桤木根瘤内生菌的分离培养  

ISOLATION AND CULTIVATION OF THE ENDOPHYTE FRANKIA FROM THE NODULE OF ALNUS NEPALENSIS

作　　者：赵之伟[1] 

机构地区：[1]云南大学生物系

出　　处：《微生物学通报》1996年第5期259-261,共3页Microbiology China

基　　金：云南省科委青年基金

摘　　要：从蒙自桤木（Ａｌｎｕｓｎｏｐａｌｅｎｓｉｓ）根瘤中分离得到４株Ｆｒａｎｋｉａ，对其进行了形态学研究，并对Ａｎ１２菌株进行了生长曲线测定，结果表明从蒙自桤木根瘤中分离Ｆｒａｎｋｉａ，除所采用的培养基和方法外，宿主植物所处的生长状态也可能是一个影响分离成败的重要因素；“Ｓ”培养基是一种较为理想的分离培养基；在离体条件下，Ｆｒａｎｋｉａ生长缓慢，约１０ｄ左右达到其生长的稳定期。Four strains of Frankia were isolated from the nodules of Alnus nepalensis.Morphological study was made on them and the growth curve of Frankia sp.AnIZ was determined in vitro.The results shown that beside the medium and the isolating method,the growthstate of the host plant would also be an importent influence factor in the successfully isolatingFrankia from the nodules of Alnus nepalensis,'S'medium was a good medium for isolating andcultivating Frankl'a.Frankl'a grown slowly in vitro, it could attain its stationary phase in about 10days.

关 键 词：弗兰克氏菌 蒙自桤木 根瘤菌 分离 培养 

分 类 号：Q939.114[生物学—微生物学]