BIOLOG细菌自动鉴定系统的应用与研究  

APPLICATION AND STUDY OF THE BIOLOG AUTOMATED BACTERIAL IDENTIFICATION SYSTEM

作　　者：谢家仪[1] 王永力[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《微生物学通报》1996年第5期264-267,共4页Microbiology China

摘　　要：利用ＢＩＯＬＯＧＭｉｃｒｏｓｔａｔｉｏｎ细菌自动鉴定系统对环境和临床来源的２０个属９０株菌进行了检测，同时以传统方法进行验证。２４ｈＢＩＯＬＯＧ系统鉴定结果：６２株革兰氏阴性菌中，５８株准确鉴定到属的水平（９３．５％），５２株准确到种的水平（８３．９％）。２８株革兰氏阳性菌中，２５株准确到属的水平（８９．３％），１１株准确到种的水平（３９．３％），总计属的水平准确率９２．２％（８３／９０），种的水平准确率７０．０％（６３／９０），其中环境来源的菌株准确率高于临床样品。本文还对某些属遇到的问题进行了讨论．Biolog Microstation identification System was used to identify 90 clinical andenviromental isolates included in 20 genus,and the results were compared with the conventionalidentifications.Of the 62 Gram-negative isolates,58(93.5%)were correctly identified at the genus level,52(83.9%)at the species level at 24 hours. Of the 28 Gram-positive isolates,25(89.3%)werecorrectly identified at the genus level,11(39.3%)at the species level at 24 hours. The overall performance of the identification was 92.2%(83/90)at he genus level and 70.0%(63/90)at thespecies level.The percentage of correct identification of enviromental isolates was higher than thatof clinical isolates.The problems encountered in the application were discussed.

关 键 词：BIOLOG系统 微生物鉴定 细菌 

分 类 号：Q93－331[生物学—微生物学] Q939.1