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作 者:赵跃武[1] 赵凤兰[1] 徐友梅[1] 胡军[1]
机构地区:[1]河南省人民医院生物治疗研究中心,河南医科大学微生物学教研室
出 处:《微生物学通报》1996年第6期339-342,共4页Microbiology China
摘 要:本实验采用超声破碎、TritonX-100处理和超速离心技术提取了鼠伤寒沙门氏菌(Salmonellatyhimurium,STM)的外膜蛋白(Outermembraneproteins,OMPs),其中脂多糖(LPS)的含量约为5%。OMPs经SDS—PAGE显示10余条蛋白带。对OMPs诱发BALB/C小鼠产生典型的迟发型变态反应(DTH)进行了检测。经腹腔免疫的BALB/C小鼠用500LD50鼠伤寒沙门氏菌(50115)攻击,100%可得到保护;用500LD50伤寒杆菌(E686)攻击,33.3%可得到交叉保护。免疫BALB/C小鼠的T淋巴细胞经尾静脉注射至非免疫小鼠,被动免疫保护率为85.7%。上述结果说明STM—OMPs能诱发BALB/C小鼠保护免疫,是一良好的保护性抗原。outer membrane proteins from Salmonella typhimurium were extracted by using supersonication,TritonX 100 and super speed centrifugation,found to be contain with 5%lipopolysacchsfide(LPS)spproximately.OMPs were Shown 10 more bands by SDS PAGE.In OMPsimmunized BALB/C mice significant level of delayed type hypersensitivity (DTH) response was dein BALB/C mice. Active immunization with OMPs induced 100%protection to a intraperitoneally(i.p) challenge with 500 LD50 of Salmonella typhimurium in BABL/C mice. In addition,33.3% crossed protection snainst challenge With up to 500 LD50 of Salmonella typhi was also achieved. Furthremore, We found that T cells from the OMPs immunized mice could transfer the protection (85.7℃) . Above resultes indicate that STM OMPs can induce more powerful and protective immunity, is good protective antigen.
分 类 号:R378.22[医药卫生—病原生物学] R392-33[医药卫生—基础医学]
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