大鼠全脑照射后早期大脑皮质中TNF-α及其受体表达的免疫组化研究  

The Immunohistochemistry Study of TNF-α and Its Receptors in Pallium of Rat Brain Following Whole-brain Irradiation

作　　者：张泉[1] 杨劲松[1] 邓翀[1] 田野[1] 

机构地区：[1]苏州大学附属第二医院放疗科,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2006年第6期915-918,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30170287);江苏省卫生厅重大科研课题资助项目(K200406);苏州大学医学发展基金资助项目(EE123504)

摘　　要：目的观察大鼠大脑全脑照射后早期放射性脑损伤大脑皮质区TNF-α、TNFR1和TNFR2的动态表达,探讨TNF-α、TNFR1和TNFR2在早期放射性脑损伤发病机制中可能的作用。方法在大鼠早期放射性脑损伤模型基础上,应用免疫组织化学方法染色标本,运用计算机图像技术计算并分析大鼠大脑放射性损伤后大脑皮质区在不同剂量、不同时间点TNF-α、TNFR1和TNFR2的动态表达。结果0 Gy组大鼠各时间点大脑皮质区TNF-α、TNFR1和TNFR2均是低水平表达,与正常组表达无显著差别(P>0.05)。在光镜下观察2 Gy组1、0 Gy组及30Gy组照射后12 h到1月不同时间点大脑皮层内TNF-α的免疫组化表达,发现抗体阳性细胞表达均在12 h达高峰(P<0.05),并可见30 Gy组数值最高(P<0.001),但10 Gy组与30 Gy组之间无显著差异(P>0.05),在24 h以后各照射组基本恢复到基础水平。同样方法观察发现2 Gy组、10 Gy组和30 Gy组的TNFR1和TNFR2的动态表达类似于TNF-α的免疫表达。结论大鼠大脑全脑照射后大脑皮质区TNF-α、TNFR1和TNFR2在24 h内快速上升快速下降,提示它们可能在放射性脑损伤中起重要作用。Objective To investigate the possible role of acute phage of brain radiation injuries by the observation of the dynamic expression of the TNF-α, TNFR1 and TNFR2 in the rat pallium. Methods A early model of brain radiation-lnduced injuries in the rat was developed. Analysis of the expression of TNF-α,TNFR1 and TNFR2 in the pallium was done at different dose and time level after whole-brain irradiation with immunohistochemistry and computer technology. Results The expression of TNF-α,TNFR1 and TNFR2 in the pallium was at：lower level in 0 Gy group. There was not significant difference between the expression of normal group and 0Gy group （P 〉0.05）, The expression of TNF-α was observed at 2 Gy 2 Gy and 30 Gy groups and different time levels with microscope. The expression of TNF-α reached the peak level at 12 h in those three groups（P 〈 0.05） the expression of TNF-α in 30 Gy group was the highest, hut there was not significant difference between the 30 Gy group and the 10 Gy group （P 〉0.05）. The expression of TNF-α in those three irradiated groups returned to the basic level after 24 h. The dynamic expressions of TNFR1 and TNFR2 were similar to that of TNF-α by the same way. Conclusion The expression of TNFα,TNFR1 and TN-FR2 in the rat pallium following radiation induced injuries increases and decreases rapidly within 24 h and shows that they may play a important role in the early pathological processes of the ionizing irradiation injury of rat brain.

关 键 词：放射性脑损伤 早期 TNF-Α 肿瘤坏死因子受体 免疫组织化学 

分 类 号：R815[医药卫生—放射医学]