内源植物激素与光敏核不育水稻农垦58S育性的关系  

RELATIONSHIP BETWEEN MALE FERTILITY AND ENDOGENOUS PHYTOHORMONES IN PHOTOPERIOD SENSITIVE GENIC MALE STERILE RICE

作　　者：赵玉锦[1] 童哲[1] 陈华君[2] 金幼菊 

机构地区：[1]中国科学院植物研究所,北京100093 [2]北京林业大学森林生物学实验中心,100083

出　　处：《Acta Botanica Sinica》1996年第12期936-941,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家高技术研究发展(863)计划资助

摘　　要：采用气相色谱－质谱联用选择离子检测（ＧＣ－ＭＳ－ＳＩＭ）技术，定性、定量分析了光敏核不育水稻农垦５８Ｓ（Ｏｒｙｚａ ｓａｔｉｖａ Ｌ． ｓｕｂｓｐ． ｊａｐｏｎｉｃａ）及对照品种“农垦５８”在长（ＬＤ）、短（ＳＤ）日照处理下，不同发育时期顶端全展叶片中内源ＩＡＡ、ＡＢＡ 和生殖器官中内源ＩＡＡ、ＡＢＡ、ＧＡ１、ＧＡ４ 的含量变化。实验结果表明：在ＬＤ 处理下，农垦５８Ｓ雌雄蕊形成期的叶片和花粉母细胞形成期的幼穗中ＩＡＡ 相继发生亏缺；农垦５８Ｓ－ＬＤ花粉母细胞形成期、单核期和扬花期，生殖器官中内源ＡＢＡ 均低于农垦５８Ｓ－ＳＤ 的含量，“农垦５８”则与此相反；扬花期不育花药中内源ＧＡ１ 和ＧＡ４ 含量剧减。据此认为：生殖器官中早期ＩＡＡ 的亏损、ＡＢＡA spontaneous male sterile rice plant (Oryza sativa L. cv. Nongken 58S)“Photoperiod sensitive Genic Male sterile Rice” has been found to be male sterile under long day cycles (LD) and fertile in short day cycles (SD). The period from secondary rachis branch and spikelet primodia to pollen mother cell formation in the process of panical development was the photoperiod sensitive stage for fertility alternation. The phenotype of this mutant was reported to be controlled by two pairs of recessive alleles. The research on relationship between the fertility alternation and phytohormone action in this mutant have been performed by Chinese scientists since 1985. In order to study the mechanism of fertility alternation in Nongken 58S, endogenous IAA, ABA, GA 1 and GA 4 in apical leaves and reproductive organs in different development stages under LD and SD conditions have been quantiatvely and qualitatively identified by GC MS SIM method. It was found that endogenous IAA in apical leaves at the stage of pistil and stamen primodia formation and in panicles at pollen mother cell stage of Nongken 58S with LD condition was deficient comparing with those in SD. Endogenous ABA level in panicles at pollen mother cell stage, in spikelets at uninucleate stage and in anthers at anthesis stage of Nongken 58S LD were lower than those in SD. ABA levels in corresponding organs and developmental stages of wild type of rice, “Nongken 58” were always higher in LD treatment than those in SD. Endogenous IAA, GA 1 and GA 4 levels in anthers at anthesis stasge of “Nongken 58” LD were increased obviously. Thus it appeared that “Nongken 58” possess stronger resistance to LD stress than Nongken 58S. It is concluded that IAA deficiency of reproductive organs at early developmental stage, ABA decrease implying poor resistance to LD stress and reduction of GA S in late developmental stages were the factors causing the anther sterility in Nongken 58S LD.

关 键 词：水稻 雄性不育 育性 光敏核不育 内源植物激素 

分 类 号：S511.035.1[农业科学—作物学]