一种新的人兽共患传染病——狐阴道加德纳氏菌病的研究 V.狐阴道加德纳氏菌菌株的血清分型研究  

A NEW ZOONOSIS——INVESTIGATION OF GARDNERELLA VAGINALIS DISEASE OF FOX V. STUDIES SEROTYPE OF GARDNERELLA VAGINALIS IN FOX

作　　者：闫新华[1] 严忠诚[1] 闫喜军[1] 栾凤英 王长凤[1] 

机构地区：[1]中国农业科学院特产研究所,吉林132109

出　　处：《微生物学报》1996年第5期373-378,共6页Acta Microbiologica Sinica

基　　金：农业部"八五"拨经费专题

摘　　要：将我国6个省(区)、13个主要狐场分离的145株狐阴道加德纳氏菌,进行抗原性、免疫原性测定,从每场分离菌中选出1～3个优良株进行血清型研究。凝集素交叉吸收试验证实,选出的26株菌可划分为3个血清型,以此3个血清型代表株制备因子血清,余下119株菌中,108株在所划分的3个血清型内,11株未能定型。在3个血清型中,Ⅰ型菌株数占定型菌数的79.1％,因而确定,Ⅰ型菌是国内狐场狐阴道加德纳氏菌主要流行型。试验还明确了从貉分离的5株、水貂分离的4株、犬分离的2株阴道加德纳氏菌也属于血清Ⅰ型。将3个血清型代表菌株制成超声抗原,经免疫琼脂扩散试验证实,各型抗原与同型或异型免疫血清均可形成一条明显的融合沉淀线,表明各型菌间有共同抗原成分。同型菌免疫琼脂扩散试验表明,各株菌形成的沉淀线完全融合,从而证实了血清分型的可靠性。145 strains Gardnerella vaginalis isolated in foxes were isolated from 13 main farms raising foxes in six provinces (regions), China, after antigenicity and immunogenicity of the strains were measured, 1 - 3 appropriate strains were selected from each farm raising foxes for serotype studies. Cross agglutinin absorption test confirmed that selected 26 strains Gardnerella vaginalis were divided into three serotypes and then the representing strains were used to produce typing serum.Among remaining 119 strains, 108 strains were typable with the typing sera, and 11 strains can't be set.Among three serotypes, serotype I made up 79.1 %of the strains.It was shown that serotype I was the principal serotype of Gardnerella vaginalis of fox in China. The test also confirmed that 5 strains of Gardnerella vaginalis isolated from racoon dog,4 strains Gardnerella vaginalis from mink and 2 strains Gardnerella vaginalis from canine also belonged to serotype I .Supersonic antigben was produced with three serotypes, representative strains.By agar immuno-diffusion test, it confirmed that the antigens of three serotypes formed a obvious blending precipitating line with the homologous or heterologous serotype antiserum. It indicated common antigen existed among all serotypes. The agar immuno-diffusion test results revealed that the precipitating line of the homologous serotype completely blended.It is our opinion that the method of serotyping is reliable.

关 键 词：狐 阴道加德纳氏菌 血清分型 感染 人兽共患病 

分 类 号：R711.73[医药卫生—妇产科学] S858.926.1[医药卫生—临床医学]