稻恶苗菌异核体三种核型核DNA的RAPD分析  

RAPD ANALYSIS OF THREE DIFFERENT COMPONENT NUCLEAR TYPES FROM FUSARIUM MONILIFORME HETEROKARYON TJ47-26

作　　者：陈希[1] 李秀玉[1] 颜耀祖[1] 

机构地区：[1]中国农业大学生物学院,北京100094

出　　处：《微生物学报》1996年第5期385-388,共4页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目

摘　　要：利用RAPD技术,对稻恶苗菌TJ47-26异核体的紫、红、白三种核型(同核体)核DNA进行比较,用80种10bp长的随机引物进行DNA扩增,共检测了基因组中近400个位点,未发现三种核型的基因组间有明显差异,表明紫、红、白三种核型的DNA碱基序列高度相似。由此推测TJ47-26异核体的三种不同核型是来自同一个核的变异体。Random Amplified Polymorphic DNA(RAPD) technique was used to compare the nuclear DNA of different component nuclear types (homokaryons) of Fusarium moniliforme TJ47-26, which were designated as W(white), R(red) P(purple) respectively and derived from the one newly isolated heterokaryon. 80 random primers with 10 base pairs were used in RAPD experiments. Nearly 400 loci were detected and no polymorphic difference was found. The banding patterns of three different nuclear types were identical by using different primers. So it is deduced that the three different component nuclear types of F. moniliforme heterokaryon TJ47-26 probably came from the variation of the same nucleus.

关 键 词：稻恶苗菌 异核体 DNA RAPD 植物病原 真菌 

分 类 号：S432.44[农业科学—植物病理学]