一株貂肠炎病毒某些特性的研究  

STUDIES ON SOME CHARACTERISTICS OF A STRAIN OF MINK INFECTIOUS ENTERITIS VIRUS

作　　者：冯锋[1] 李天宪[1] 赵林[1] 马志勇 陈溥言[2] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071 [2]南京农业大学动物医学院,南京210095

出　　处：《微生物学报》1996年第6期468-471,共4页Acta Microbiologica Sinica

摘　　要：采用猫肾传代细胞FK增殖华东地区分离的貂传染性肠炎病毒(mink infectious enteritis virus,MEV-S_1)能产生明显的细胞病变。浓缩的病毒液经Sepharose-4B柱层析提纯处理后置电镜下观察,可见典型的病毒粒子。经蛋白酶K、SDS裂解病毒,用苯酚-氯仿法抽提病毒核酸,二苯胺试验和酶消化试验表明该病毒核酸为DNA类型。吖啶橙染色试验表明该DNA为单链。甲酰胺法进行核酸分子展层,病毒核酸呈线状,长度为1.5～2.0/μm。A strain of mink infectious enteritis virus that was isolated from the east district of China can be bred on FK cell. Concentrated viruses were purified by using Sepharose-4B chromatography. The size of the virus was about 20 - 22nm by electron microscope. Viral nucleic acid was extracted from pure virus by using SDS-proteiase K-Phenol. Tests with diphenylamine, acridine orange and the curve of thermal dentuation, etc. showed that the virus had a single-stranded DNA. The molecular weight of the ssDNA was from 1.5 ×106 to 2.0 ×106d determined by length of the virul nucleic acid.

关 键 词：动物病毒 貂传染性肠炎 病毒 细胞病变 特性 

分 类 号：S852.65[农业科学—基础兽医学] S858.953[农业科学—兽医学]