微菌落观察法快速检测和鉴别结核杆菌培养物的研究  

MICROCOLONY OBSERVATION FOR RAPID DETECTION AND IDENTIFICATION OF CULTURES OF MYCOBACTERIUM TUBERCULOSIS

作　　者：匡铁吉[1] 宋萍[1] 金关甫[1] 王仲元[1] 朱建华[1] 刘金伟[1] 秦振伦 李伟霞[1] 陈洪斌 

机构地区：[1]解放军三○九医院,北京100091

出　　处：《微生物学报》1996年第6期472-475,共4页Acta Microbiologica Sinica

摘　　要：将取自肺结核患者的219例痰标本接种匡氏琼脂平板,共分离到112例结核菌生长物。其中培养法104例(92.8％)阳性,微菌落观察法108例(96.4％)阳性,微菌落法阳性率稍高。培养法和微菌落法阳性标本首次检出时间分别为18.6d和11d,微菌落检出时间更短(P<0.01)。常规菌型鉴别方法与微菌落法对结核杆菌菌型鉴别的符合率为99％。219 sputa were seeded on Kuang's agar plates. A total of 112 isolates of Mycobacterium tuberculosis were detected in 219 specimens. Of these 112 isolates, 104 (92.8%) were detected in Kuang's agar medium arid 108(96.4%) were detected by microcolony observation. The detection time of microcolony observation and culture method needed 11 and 18.6 days respectively. The detection time of microcolony method is much shorter (P< 0.001). The results of conventional tests of different species of Mycobacterium and microcolony differentiation were identical in 99% of isolates of Mycobacterium tuberculosis.

关 键 词：结核杆菌 微菌落 琼脂培养基 

分 类 号：R378.911[医药卫生—病原生物学]