生物素—链霉亲和素系统在检测MHV抗体中的应用  

Application of Biotin-Streptoavidin System in Detection of MHV Antibodies

作　　者：屈霞琴[1] 姜文琴[1] 钱琴[1] 应贤平[1] 潘振业[1] 陈天培[1] 

机构地区：[1]卫生部上海生物制品研究所,上海200051

出　　处：《上海实验动物科学》1996年第3期142-144,共3页Shanghai Laboratory Animal Science

摘　　要：采用生物素—链霉亲和素系统(BSA)试剂建立测定MHV抗体的BSA-ELISA法,并与SPA-ELISA法比较。结果表明,前者本底极低,敏感性比后者提高4倍,阳性检出率提高1倍,且经阻断试验证明其特异性高。因此,该法在检测实验动物的特异性抗体方面,具有很大的应用潜力。Biotin carboxyl anti - antibody and enzyme - labled streptoavidin were used in the indirect enzyme - linked immunosorbent assay (ELISA) for the detection of antibodies to mouse hepatitis viruses (MHV) in mice. The results showed that it's sensitivity was four times higher than that of the ELISA with enzyme -labled staphylococcal protein A, and it's specificity was also better than the latter.

关 键 词：小鼠肝炎病毒 生物素 链霉亲和素 实验动物 抗体 

分 类 号：S858.926.5[农业科学—临床兽医学] R－332[农业科学—兽医学]