孕牛外周血中胎儿Y-特异性序列的检测  

Detection of Bovine Fetal Y-specific Sry Sequence from Maternal Blood

作　　者：杨建民[1] 王骊山[1] 姜小军 蒋耀青 刘连瑞[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《生物工程学报》1996年第3期307-310,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金资助

摘　　要：根据公牛Sry特异性序列设计两对寡核苷酸引物。并对20份妊娠晚期母牛外周血DNA样品进行PCR扩增,结果有9份样品检测出有Sry片断(阳性),其余11份为阴性。经分娩牛犊性别验证,在20份被检测的样品中,有16份PCR检测结果与犊牛实际性别相符,其余4例不符。我们的实验结果说明,胎儿细胞可以进入到孕牛的外周血中去。Two pairs of primers were designed based on sequence of bovine Sry and applied to assay of Y-specific DNA sequence from nmaternal blood using the polymerase chain reaction (PCR). Of the total twenty DNA samples from peripheral blood of late pregnant cow,nine appeared positive with male specific sequence. The fetal sex predicted by PCR analysis of maternal blood DNA accorded with the sex of babies on delivery in 16 cows (the remaining four were negative). Our results showed that bovine fetal cells can enter the maternalblood of pregnant cow.

关 键 词：牛 孕牛 胎儿 性别 外周血 Y-特异性序列 

分 类 号：S823.2[农业科学—畜牧学]