康宁木霉(Trichoderma koningii)SMF2分泌的peptaibols类抗菌肽Trichokonins抑菌活性研究  

Antimicrobial activities of Trichokonins: Peptaibol-like antimicrobial peptides produced by Trichoderma koningii SMF2

作　　者：解树涛[1] 宋晓妍[1] 石梅[1] 陈秀兰[1] 孙彩云[1] 张玉忠[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《山东大学学报（理学版）》2006年第6期140-144,共5页Journal of Shandong University(Natural Science)

基　　金：教育部新世纪优秀人才支持计划资助项目(NCET-04-0637);山东省科技发展计划资助项目(030304);山东省优秀中青年科学家奖励基金资助项目(2004BS06001)

摘　　要：Peptaibols是一类来源于土壤微生物,由非核糖体合成酶(nonribosomal peptide synthetase,NRPS)合成的富含α-氨基异丁酸(-αaminoisobutyric acid,Aib)的抗菌肽的总称.Trichokonins属于长链peptaibols第一亚家族,其对普通金黄色葡萄球菌和1株临床分离的多抗金黄色葡萄球菌的最低抑菌浓度(minimum inhibitory concentration,MIC)均为50μg/mL,但时间杀菌曲线表明后者对MIC浓度的Trichokonins耐受性明显大于前者.在含50μg/mL浓度Tri-chokonins的培养基中连续转接20次后普通金黄色葡萄球菌和多抗金黄色葡萄球菌对Trichokonins的敏感性都明显降低,MIC值均提高到200μg/mL以上,表明金黄色葡萄球菌对Trichokonins产生了明显的抗性.这与目前普遍认为的抗菌肽不易产生抗性的观点不一致,其抗性产生的具体机制有待于进一步的研究.Peptaibols is a kind of Aib-riching pepfide antibiotics mostly produced by soil fungi and synthesized by nonribosomal peptide-synthetase （NRPS）. Trichokonins, with full length of 20 amino acids, belong to the first subfamily of peptaibols. The minimal inhibitory concentration （MIC） of Trichokonins against ordinary Staphylococcus aureus and a multi-drug resistant Staphylococcus aureus strain are both 50 μg/mL. It can be observed from the time-killing curves that the multi-drug resistant strain shows a higher survival rate in the presence of 50 μg/mL Trichokonins. When they were inoculated for 20 times in 50 μg/mL riftchokonins, the sensitivity of both strains to Trichokonins decreased greatly, and the MICs increased to higher than 200 μg/mL. The results indicate that both strains become resistant to Trichokonins after the treatment. The mechanism of the＇ resistance requires further experimentation for its disagreement with the existing views.

关 键 词：康宁木霉SMF2 PEPTAIBOLS Trichokonins MIC 时间杀菌曲线 

分 类 号：Q93[生物学—微生物学]