高效液相色谱法测定骨疏灵提取物中淫羊藿苷含量  

Content Determination of Icariin in Gushuling Extract by HPLC

作　　者：王淑梅[1] 高振强[2] 王川平[1] 孙倩[1] 刘倩[1] 李宏辉[1] 

机构地区：[1]河北医科大学第二医院,河北石家庄050000 [2]河北医科大学第三医院,河北石家庄050051

出　　处：《中国中医药信息杂志》2007年第1期51-52,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的测定骨疏灵提取物中淫羊藿苷的含量。方法采用高效液相色谱法,色谱柱为Luna C18(250mm×4.6mm,5μm),流动相为乙腈-水(30∶70),检测波长270nm。结果淫羊藿苷的线性范围为0.416～2.496μg,回归方程为Y=2.0×106X-53810(r=0.9999)。结论所用高效液相色谱法简便、快速、准确,可用于测定骨疏灵微球提取物中淫羊藿苷的含量。Objective To determine the content icariin in the extract of Gushuling. Methods HPLC method was performed on Luna C18 column （250 mm×4.6 mm, 5μm）. The mobile phase consisted of acetonitrile- water （30 ; 70） and the UV detective wavelength was set at 270 nm. Results The curve of icariin was linear within 0.416-2.496μg, Y=2.0×10^6X-53 810 （r=0.999 9）. Conclusion HPLC method is simple, rapid and accurate, and it is suitable for ananlvsis of icariin content in extract of Gushuling.

关 键 词：高效液相色谱法 骨疏灵 淫羊藿苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]