人类白细胞抗原等位基因与HBV宫内感染关系  

Association between HLA-DR alleles and HBV intrauterine infection

作　　者：冯永亮[1] 王素萍[1] 史晓红[1] 魏俊妮[1] 赵小嘉[2] 安珊珊[2] 陈晓[2] 麻春林[2] 张昌琳[2] 

机构地区：[1]山西医科大学流行病学教研室,太原030001 [2]山西医科大学

出　　处：《中国公共卫生》2007年第1期75-77,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30070699);山西医科大学学生创新基金项目

摘　　要：目的研究人类白细胞抗原(HLA)-DR3、DR7、DR13、DR53等位基因与乙型肝炎病毒(Hepatitis Bvirus,HBV)宫内感染的关系,探讨HBV宫内感染的遗传易感性,确定HBV宫内感染的易感基因和保护基因。方法采用聚合酶链反应/序列特异性引物(PCR-SSP)技术检测HBsAg阳性孕妇及其新生儿各187例外周静脉血血块中HLA-DR3、DR7、DRl3、DR53等位基因型分布及频率。结果(1)宫内感染组孕妇HLA-DR3基因频率为24.14%(7/29),显著高于非宫内感染组孕妇的6.33%(10/158)(P=0.007)。其余各表型比较,差异无统计学意义(P>0.05)。(2)宫内感染组新生儿HLA-DR3基因频率为17.24%(5/29),显著高于非宫内感染组新生儿的5.06%(8/158)(P=0.033)。其余各表型比较,差异无统计学意义(P>0.05)。(3)母婴HLA-DR3同阳性在宫内感染组与宫内未感染组间比较差异有统计学意义(P=0.049);母婴HLA-DR7、DR13、DR53同阳性在宫内感染组与宫内未感染组间比较,差异无统计学意义(P>0.05)。结论HBsAg阳性孕妇及其胎儿同时或任一携带HLA-DR3,易导致HBV宫内感染;HLA-DR3是HBV宫内感染的易感基因。Objective To study the association between HLA- DR3, DR7, DR13, DR53 and HBV intrauterine infection to probe into the susceptibility to intrauterine infection of hepatitis B virus and to find its predisposing genes and protecting genes. Methods HLA - DR3, DR7, DR13, DR53 were detected by the technique of polymerase chain reaction - sequence specific primers（PCR - SSP） in maternal and neonatal blood clot. Results （1） The frequency of HLA - DR was 24.14 96 in women with intrauterine infection, which was higher than that （ 6.33 % ） in women without intrauterine infection （ P = 0. 007）. There was no association between HLA- DR7, DR13, DR53 positive in pregnant women and HBV intrauterine infection（ P 〉 0.05）. （2） The frequency of HLA- R of newborns was 17.24 % in women with intrauterine infection, which is higher that （5.06%） in women withotll infection（P = 0. 033）. There was no association between HLA - DR7, DR13, DR53 positive in newborns and HBV intrauterine infection（ P 〉 0.05 ）. （3） There was significant association between HLA - DR3 concordance and HBVintrauterine infection（P = 0. 049）. There was no association between DR7, DR13, DR53 concordance and HBV intrauterine infection（P 〉 0.05 ）. Conclusion HLA - DR3 carriers of pregnant women and their newborns have susceptibility to HBV intrauterine infection. HLA- DR3 is the predisposing gene of HBV intrauterine infection.

关 键 词：乙型肝炎病毒 宫内感染 人类白细胞抗原DR等位基因(HLA-DR) 

分 类 号：R174.6[医药卫生—妇幼卫生保健]