大气污染物对大鼠肺炎性细胞因子影响  

Effects of air pollutants on pulmonary inflammatory cytokines in rats

作　　者：肖纯凌[1] 王任群[1] 赵肃[1] 王灿[1] 金焕荣[1] 

机构地区：[1]沈阳医学院,沈阳110034

出　　处：《中国公共卫生》2007年第1期83-84,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30471425)

摘　　要：目的研究大气污染物对大鼠肺炎性细胞因子的影响，探讨大气污染对呼吸系统炎性损伤的机制。方法48只Wistar大鼠，体重200～240g，随机分为实验组（1d组、7d组和30d组）和对照组。实验组分别进行可吸入颗粒物（PM10）染尘，SO2、NO2和CO染毒。PM10染尘采用气管注入法，灌注1ml含15mgPM10生理盐水混悬液；染毒采用静态吸入SO2、NCh、CO空气混合气，浓度分别为15，12，400mg/m^3，每天吸入2h，分别吸入1，7及30d；对照组大鼠气管注入1ml生理盐水并吸入正常空气。分别于染毒后1．7，30d进行支气管肺泡灌洗。观察灌洗液中自细胞总数及其分类；采用ELISA法测定支气管肺泡灌洗液（BALF）中炎性细胞因子水平。结果实验组大鼠BALF中，白细胞总数、中性粒细胞百分比明显升高，与对照组比较差异有统计学意义（P〈0．05或P〈0．01）。1d组大鼠BALF中。肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）及白细胞介素-10（IL-10）水平均明显高于对照组（P〈0．05），各炎性细胞因子间有较好的相关性。结论大气污染物可引起大鼠肺促炎细胞因子与抗炎细胞因子表达失衡，造成肺组织的炎性损伤。Objective To study the effects of air pollutants on pulmonary inflammatory cytokines in rats, and to explore the mechanism of air pollution on inflammatory injury of respiratory system. Methods Fourty - eight Wistar rats （200- 240 g） were randomly divided into treated groups （ 1 d, 7 d, and 30 d groups） and control group. The tested rats were respectively exposed to PM10 of 15 mg in 1 ml saline by intratracheal instillation, and then were exposed to mixed gases of SO2, NO, and CO （15, 12, and 400 mg/m^3 respectively） by static inhalation 2 h per day for 1 d, 7 d and 30 d respectively. One ml saline was instilled and general air was inhaled by rats of the control group. Results The counts of white blood cell （WBC） and the percentages of neutrophil （Neu） in treated groups were significantly higher than those in the control group （P 〈 0.05 or P 〈 0.01）. The levels of TNF-α, IL-6, IL-8, and IL-10 in rats of one-day group were significantly higher than those in the control group （P 〈 0.05）. There were positive relationships among cytokines. Conclusion Air pollutants can induce the imbalance on the expression of pro-and anti-inflammatory cytokines and result in inflammatory injury of respiratory system.

关 键 词：大气污染物 支气管肺泡灌洗液 细胞因子 

分 类 号：R994.6[医药卫生—毒理学]