玉米果穗结实习性遗传特点及其SSR标记分析  

Inheritance of Fructification Habit and Its Relative SSR Markers in Maize

作　　者：孟昭东[1] 李文才[2] 孙琦[2] 张发军[2] 丁照华[2] 汪黎明[2] 郭庆法[2] 王洪刚[1] 

机构地区：[1]山东农业大学农学院,山东泰安271018 [2]山东省农业科学院玉米研究所/国家玉米改良分中心,山东济南250100

出　　处：《作物学报》2007年第1期90-96,共7页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)重大专项项目(2002AA207008);农业部农业结构调整重大技术研究专项项目(04-03-03B)

摘　　要：为了探明玉米果穗结实习性的遗传特点,选用有限结实特点的lx01-3和无限结实特点的wx04-1玉米自交系进行杂交。在30 000株hm-2的种植密度下,分析了两亲本自交系及其F1、F2、BC1和BC2植株的果穗结实特性。结果表明,F1代植株的果穗均为无限结实类型,无限结实类型为显性;无限结实与有限结实类型的分离比例,在F2群体中符合15∶1,在BC1群体中符合3∶1,表明玉米果穗的结实习性可能是由2对存在重叠作用的基因决定的。利用F2分离群体分组法进行SSR标记分析发现,引物bnlg1601和umc1663扩增的特异产物带与控制结实习性的基因存在密切的连锁关系,进而将控制玉米果穗结实习性的2个基因初步定位于玉米的第3和第8染色体。Barren tip phenomenon of the ear in maize （ Zea mays L. ） caused the yield loss and affected the quality of commodity grain remarkably. This phenomenon is generally regarded as a quantitative character easily affected by planting density, fertilizer and water supply, light and temperature conditions, etc. However, we found that this phenomenon varied a lot among varieties and inferred that it was caused by the difference of maize fructification habit. Based on this understanding, maize varieties were classified into two types, definite fructification type （ab. DF type） and indefinite fructification type （ab. IDF type）, mainly according to the development states of the kernels on tip part of the ear. In order to probe the inheritance pattern of maize ear fructification habit, two inbred lines, lx01-3 （DF type） and wx04-1 （IDF type）, and their F1 , F2, BC1, and BC2 populations were analyzed on the ear fructification type under a planting density of 30 000 plants per hectare in this experiment. The results showed that all ears of F1 were IDF type which means IDF type is dominant over DF type; the ratios of IDF type versus DF type ears followed a 15 ： 1 in F2 and a 3 ： 1 in BC1 population while all ears were IDF types except one in BC2 population, which indicates the trait of fructification habit probably controlled by two pairs of duplicate dominant genes. Two SSR markers, bnlg1601 and umc1663, were found closely linking with the genes controlling the trait of maize fructification habit. Thereby, the two genes were preliminarily located on chromosomes 3 and 8 respectively.

关 键 词：玉米 结实习性 重叠作用 SSR标记 

分 类 号：S513[农业科学—作物学]