甘蓝型油菜NCa CMS育性相关候选线粒体基因及其恢复基因的转录调控  

Candidate Sterility-Related Mitochondrial Genes of NCa CMS in Brassica napus L. and Its Transcription Regulated by Restorer Gene

作　　者：危文亮[1] 王汉中[1] 刘贵华[1] 

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物遗传改良重点开放实验室,湖北武汉430062

出　　处：《作物学报》2007年第1期102-106,共5页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2002AA207009);武汉市青年科技晨光计划项目(20035002016-36);农业部油料作物遗传改良重点实验室开放课题

摘　　要：用10个线粒体基因为探针,对NCa不育系、保持系和可育F1幼蕾的线粒体RNA进行了Northern检测。结果表明,atp6、atp1、cox1c、ox2、cobr、rn5S和rrn26S等7个线粒体基因探针在不育系、保持系、可育F1中的转录没有差异,只有orf222、orf139和atp9等3个探针检测到转录本的差异。orf222和orf139分别在不育系和可育F1中产生相同大小和丰度的转录本,但是在保持系中没有检测到转录本;orf222检测到的3条转录本分别为1.1、0.9和0.6 kb,orf139检测到0.8和0.6 kb 2条带。atp9探针在不育系和保持系中都检测到1条0.6 kb转录本,而在可育F1中检测到0.6和1.2 kb的转录本。NCaCMS不育的形成可能与orf222、orf139和atp9基因的表达有关。讨论了恢复基因在F1育性恢复过程中对育性相关候选基因的可能调控方式。Previous studies have verified that NCa CMS was a novel CMS and would be rather important for improving cytoplasm monotony in rapeseed hybrid utilization, but the transcriptional pattern of NCa CMS was not investigated yet. So in this paper, Northern blotting analysis was conducted with mitochondrial RNA from floral buds of NCa CMS, maintainer line and fertile F1 using 10 mitochondrial genes as probes. Transcripts of seven probes of atp6, atp1, cox1, cox2, cob, rrnSS, and rrn26S, showed no diversity among floral buds of these three materials, which indicated that the expression of these probes has no relationship with NCa sterility. However, differences in transcriptional patterns were observed when using orf222, orf139 and atp9 as probes. Three transcripts of orf222 were detected in the floral buds of NCa CMS and fertile F1 while no transcript was detected in those of the maintainer line. The transcription of orf139 was similar to that of orf222 but only two transcripts of 0.8 and 0.6 kb. The atp9 probe detected a single transcript of 0.6 kb in NCa CMS and maintainer line but an additional transcript of 1.2 kb in fertile F1 . The results suggested that the expression of orf222, orf139 and cap9 might be associated with NCa sterility. And how the restorer gene regulates the expression of these candidate sterility-related mitochondrial genes and then causes fertility restoration in fertile F1 is not clear and detail research about the regulation mechanism is underway.

关 键 词：甘蓝型油菜 细胞质雄性不育 线粒体基因表达 恢复基因 转录调控 

分 类 号：S565.4[农业科学—作物学]