节律蛋白mPERl在NIH3T3细胞增殖和迁移中的作用  

Effect of mPERl on cell growth and migration of NIH3T3 cells

作　　者：汪宇辉[1] 刘延友[1] 江舟[1] 冀治鸿[1] 杨波[1] 胡丽娟[1] 鲁芳[1] 王正荣[1] 

机构地区：[1]四川大学华西医学中心基础医学与法医学院,四川成都610041

出　　处：《西部医学》2007年第1期3-5,共3页Medical Journal of West China

基　　金：国家自然科学基金(No.30070278;No.30470623)

摘　　要：目的研究PER 1蛋白对N IH 3T 3细胞增殖和迁移的影响。方法将重组表达质粒pcDNA 3.1/m PER 1转染入N IH 3T 3细胞中,并以未转染的N IH 3T 3细胞为对照,利用M TT法检测细胞增殖的改变。利用RT-PCR技术和W esternb lot检测节律蛋白m PER l对细胞迁移关键性蛋白基质金属蛋白酶2(MM P-2)表达的影响。结果M lTT法显示PER 1表达上调可以抑制N IH 3T 3细胞增殖,RT-PCR技术和W estern b lot检测显示在N IH 3T 3细胞中,当节律蛋白m PER 1表达上调时,细胞迁移关键性蛋白MM P-2在RNA和蛋白水平的表达较对照组降低。结论上调N IH 3T 3细胞内的PER 1蛋白表达能抑制细胞增殖以及细胞迁移的关键性MM P-2在RNA和蛋白水平的表达。Objective To study the function of mPERI on cell growth and migration of NIH3T3 cells. Methods The peDNA3, l/roPER1 constructed plasmid was transferred into NIH3T3 cells by the Lipofectaml TM 2000 reagent and con- trolled by the NIH3T3 cells without peDNA3.1/mPERI, The cell growth of these two groups was detected by MTT and the expression of cell migration proteln-MMP-2 was analyzed by RT-PCR and Western blot. Results NIH3T3 cell proliferation was inhibited when mPER1 expression was up--regulated. The level of MMP-2 expression in NIH3T3 cells was decreased after transfection with the pcDNA3.1/mPERI plasmid. Conclusion The cell growth of NIHST3 and the expression of cell migration protein MMP-2 will be decreased when mPERI expression is up-regulated.

关 键 词：PERL MMP-2 细胞增殖 细胞迁移 

分 类 号：R966[医药卫生—药理学] Q789[医药卫生—药学]