颈动脉损伤大鼠新生内膜增生与局部平滑肌细胞的增殖  

Proliferation of focal smooth muscle cells and intimal hyperplasia after injury of carotid artery in rats

作　　者：陈凤英[1] 郭艳红[2] 高炜[2] 

机构地区：[1]内蒙古医学院附属医院急救中心,内蒙古自治区呼和浩特市010050 [2]北京大学第三医院心内科,北京市100083

出　　处：《中国组织工程研究与临床康复》2007年第2期222-225,T0001,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察大鼠颈动脉损伤后高同型半胱氨酸血症对新生内膜形成和局部炎症因子单核细胞趋化因子1、细胞间黏附分子1表达及局部平滑肌细胞增殖的影响。方法:实验于2004-10/2005-02在北京大学第一临床医院心血管研究所完成。①Wistar雄性大鼠50只,采用随机数字法分为蛋氨酸组:1g/(kg·d)L-蛋氨酸灌胃;对照组:饮用水灌胃,每组25只。4周后检测两组血浆同型半胱氨酸浓度,并行左颈动脉球囊拉伤,分别于3,7,14,28d取材,检测血管损伤后新生内膜增生和局部单核细胞趋化因子1、细胞间黏附分子1表达情况。结果:①两组大鼠血浆同型半胱氨酸浓度:蛋氨酸组血浆同型半胱氨酸浓度明显高于对照组[(106.19±19.75),(4.90±0.10)μmol/L]。②两组大鼠血管组织形态学观察结果:苏木精-伊红染色血管损伤后7d可见内膜增生,14,28d蛋氨酸组新生内膜增生厚度比对照组增加36%,33%;新生内膜面积增加41%,30%;内膜面积/中膜面积增加36%,21%;管腔面积减少47%,61%;两组间比较差异均有显著性,但中膜面积无显著性差别。蛋氨酸组14d血管内膜和中膜平滑肌细胞中增殖细胞核抗原阳性颗粒百分比是对照组的1.7倍和2.3倍[(84.40±9.05)%比(49.00±4.51)%,P<0.01;(53.30±5.25)%比(23.70±6.17)%,P<0.01]。未损伤的右颈总动脉两组血管内膜及中膜增殖细胞核抗原阳性颗粒数小于0.1%。蛋氨酸组血管损伤局部单核细胞趋化因子1、细胞间黏附分子1表达增强,单核细胞趋化因子1主要分布在新生内膜和中膜,细胞间黏附分子1主要分布在内皮下和新生内膜,3d,7d明显,持续28d。结论:高同型半胱氨酸血症上调血管损伤局部炎症因子单核细胞趋化因子1、细胞间黏附分子1表达,促使局部平滑肌细胞增殖,使新生内膜增生。AIM： To study the effects of hyperhomocysteinemla on the expressions of focal inflammatory factors such as monocyte chemoattractant protein-1 （MCP-1） and intercellular adhesion molecule-1 （ICAM-1） as well as intimal hyperplasia （IH） and focal smooth muscle cells （SMCs） in a rat model of carotid artery injury. METHODS： The experiment was conducted in the Institute of Cardiology, Peking University First Hospital from October 2004 to February 2005.①Fifty male Wistar rats ware divided randomly into two groups （n =25 per group）. Control group was Infused with tap water in stomach, and the experiment group was treated with tap water containing 1 g/kg L-methionine via Intragastrically injection. After 4 weeks of their respective feed. all animals underwent left common carotid artery balloon denudation, and 3, 7, 14 and 28 days later, the plasma Homocystelne （Hcy） concentrations were measured. The morphology of carotid artery IH was studied. The expressions of MCP-1 and ICAM-1 were also examined. RESULTS： ①The levels of plasma Hcy in experiment group were significantly higher than that of control group [（106.19±19.75）, （4.90±0.10） μmol/L].②Morphology of carotid artery： After 7 days of hematoxylin-eosin staining, IH developed in all balloon-injured arteries of two grOups. At days 14 and 28, the intimal thickness, intimal area and intima/media area ratio were Increased by 36% and 33%, 41% and 30%, 36% and 21%, respectively, whereas luminal area was reduced by 47% and 61%, separately in the experiment group compared with the control group. The difference was significant in above indexes except media area between the two groups. At day 14, the percentages of intima and media SMC proliferation cell nuclear antigen positive particles of the experiment group were also significantly promoted by 1.7-fold and 2.3-fold compared with the control group, which was less than 0.1% [（84.40±9.05）% vs. （49.00±4.51）%, P 〈 0.01; （53.30±5.25）% vs. （23.70±6.17）%, P 〈

关 键 词：高半胱氨酸 肌 平滑 炎症趋化因子类 胞间粘附分子 

分 类 号：R329.41[医药卫生—人体解剖和组织胚胎学]