高效液相色谱法测定人血浆中阿立哌唑浓度  

Determination of Aripiprazole in Human Plasma by HPLC

作　　者：刘伟忠[1] 黄伟侨[2] 黎曙霞[2] 王广发[1] 王华成[1] 

机构地区：[1]广州市精神病医院,国家药品临床研究基地,广州市510370 [2]中山大学附属第一医院药学部,广州市510080

出　　处：《中国药房》2007年第2期111-113,共3页China Pharmacy

基　　金：广东省社会发展领域科技计划项目基金资助(编号:53060)

摘　　要：目的:建立以高效液相色谱法测定人血浆中阿立哌唑浓度的方法。方法:血浆样品经液-液提取后,采用高效液相色谱法检测。色谱柱为C18,流动相为0．03mol·L-1醋酸铵-乙腈(34:66),流速为0．8mL·min-1,柱温为40℃,检测波长为257nm,灵敏度为0．01 AUFS。结果:阿立哌唑检测浓度线性范围为5．0～600．0ng·mL-1(r=0．999 5),提取回收率均大于90％。结论:该方法灵敏、准确、快速,适用于人血浆中阿立哌唑浓度的检测。OBJECTIVE： To establish a HPLC method for the determination of aripiprazole in human plasma by HPLC. METHODS：The plasma sample was determined by HPLC after subjected to liquid-liquid extraction,The separation was carried out on C18 column with clolumn temperature at 40℃. The mobile phase consisted of 0.03mol·L^-1 ammonium acetate-acetonitrile（34 ： 66） with a flow rate of 0.8mL·min^-1 and detective wavelength of 257nm,The sensitivity was 0.01 AUFS. RESULTS：The linear range of aripiprazole was 5.0-600.0ng·mL^-1（r = 0.999 5）. The recovery rate was above 90 %. CONCLUSION ：The method is sensitive,accurate and rapid,and suitable for the determination of aripiprazole in human plasma.

关 键 词：阿立哌唑 药动学 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学] R971.4[医药卫生—药学]