盐酸戊乙奎醚对腹腔感染败血症小鼠肝脏病理组织学改变及生存率的影响  

Effects of penehyclidine hydrochloride on liver pathology and survival rate in septic mice

作　　者：詹佳[1] 王焱林[1] 贾宝辉[1] 雷利荣[1] 王成夭[1] 

机构地区：[1]武汉大学中南医院麻醉科,430071

出　　处：《江苏医药》2007年第1期45-46,F0003,共3页Jiangsu Medical Journal

基　　金：湖北省卫生厅科研基金项目(2005JX2C61)

摘　　要：目的观察盐酸戊乙奎醚(PHC)对腹腔感染败血症小鼠肝脏病理组织学改变及生存率的影响。方法采用盲肠结扎并穿孔(CLP)复制败血症模型。55只小鼠随机分为对照组10只(假手术组,)、CLP组15只、PHC0·6mg/kg组10只、PHC0·45mg/kg组10只和PHC0·3mg/kg组10只。模型制备成功后6h取肝脏进行光镜和电镜检查,并观察48h内小鼠的生存情况。结果48h内CLP组、PHC0·6mg/kg组、PHC0·45mg/kg组和PHC0·3mg/kg组小鼠生存率分别为6·7%、30·0%、70·0%和50·0%。光镜下CLP组肝细胞肿胀,肝血窦扩张。电镜下可见CLP组肝细胞凋亡,细胞器明显破坏。PHC0·45mg/kg组肝组织病理改变较CLP组明显减轻。结论0·45mg/kgPHC可以改善腹腔感染败血症小鼠肝脏的病理组织学改变,提高其生存率,减轻严重感染导致的机体损伤。Objective To investigate the effects of penehyelidine hydroehloride （PHC） on liver pathology and survival rate in mice after a cecal ligation and puncture. Methods Mice models of abdominal infection were established by a cecal ligation and puneture（CLP）. Fifty-five healthy female mice were randomly divided into control group（sham group, n=10）, CLP group（n=15） , PHC 0. 6 mg/kg group（n= 10）, PHC 0. 45 mg/kg group（n= 10） and PHC 0. 3 mg/kg group（n= 10）. The livers of CLP group and PHC 0. 45 mg/kg group were sampled at 6 h after CLP for light and electronic microscopy examination. The survival rate of each group was observed during 48 hours. Results Compared with control group, there were obvious changes in histology of liver tissues in CLP group. Within 48 hours, the survival rates of CLP group,PHC 0. 6,0. 45 and 0. 3 rng/kg groups were 6. 7%, 30. 0%, 70.0% and 50%, lespectively. Compared with CLP group, PHC relieved histological changes of liver tissues and increase survival rates （P〈0. 05）. Conclusion PHC 0. 45 mg/kg group may improve liver histology and increase survival rate of sepsis mice.

关 键 词：盐酸戊乙奎醚 败血症 病理学 生存率 

分 类 号：R971[医药卫生—药品]