超高压力对流感病毒生物活性的影响  

Effects of high hydrostatic pressure on biological activity of influenza viruses

作　　者：范东瀛[1] 姜述德[2] 陈俊英[2] 蒋燕军[2] 邱丰[2] 李卫东[2] 廖国阳[2] 

机构地区：[1]首都医科大学基础医学院微生物教研室,北京100069 [2]中国医学科学院中国协和医科大学医学生物学研究所,昆明650118

出　　处：《重庆医学》2007年第1期57-59,共3页Chongqing medicine

基　　金：云南省自然科学基金面上项目资助(200420070M);2003年度云南省科技厅创新人才引培项目资助(2003py07)

摘　　要：目的研究超高压力对流感病毒影响。方法通过血凝素、感染性和RP-PCR等检测不同压力处理后流感病毒的变化。结果250MPa处理1h病毒感染力下降了4～5log10TCID50／ml，血凝效价变化不大；350MPa以上，病毒感染性完全消失，血凝效价也明显降低。但400MPa压力下仍可扩增出流感病毒组。结论流感病毒在250MPa压力下血凝素抗原损伤小，基因耐高压能力较强。Objective To study effects on the biological activity of influenza viruses by high hydrostatic pressure. Methods The influenza viruses,which were treated with 250 MPa,350 MPa and 400 MPa pressures, were tested by hemagglutin, viral infective titration and RT-PCR. Results Influenza viruses were completely inactivated at greater than 350 MPa within lh,but hemagglutin titers of the viral suspends were decreased consumedly. At 250 MPa pressure, infective viruses were reduced by more than 4 log10TCID50/ml, but the hemagglutins were destroyed lightly. The genomes of influenza viruses were integrality at 400 MPa by RT-PCR. Conclusion Hemagglutins were destroyed lightly by 250 MPa pressure and genome of influenza viruses was a better compression resistance.

关 键 词：超高压力 流感病毒 灭活 

分 类 号：R373[医药卫生—病原生物学]