缬沙坦对2型糖尿病大鼠肾脏抗氧化应激作用机制的研究  

Mechanic Effect of Valsartan on Antioxidative Stress of Kedney in Type 2 Diabetic Rats

作　　者：黄娟[1] 韩敏[1] 刘慎微[1] 刘晓城[1] 徐钢[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《中国药师》2007年第1期3-5,共3页China Pharmacist

基　　金：国家自然科学基金(编号:30370657);教育部"新世纪优秀人才支持计划"(编号:MCET-04-0712);湖北省自然科学基金项目(编号:2006ABB022)。

摘　　要：目的:观察缬沙坦对实验性2型糖尿病大鼠肾脏病变的保护作用及其机制。方法:链脲佐菌素(STZ)诱导的糖尿病肾病大鼠模型随机分为对照组糖尿病组及治疗组(缬沙坦10mg·kg^(-1)·d^(-1)),治疗6周后,比较各组大鼠肾组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,同时检测各组大鼠血糖、血肌酐、尿素氮、24h尿蛋白定量等。采用RT-PCR方法检测P22phoxmRNA表达。结果:治疗组较非治疗组明显改善尿白蛋白、尿素氮水平和肾脏肥大指数。肾脏MDA含量明显下降(P＜0．05),SOD活性显著上升(P＜0．05)。p22phox的mRNA相对含量在糖尿病组为(0．875±0．94),明显高于单切时照组(0．297±0．067)(P＜0．01),在治疗组为(0．484±0．064),较糖尿病组显著降低(P＜0．01),但仍高于对照组(P＜0．05)。结论:缬沙坦对2型糖尿病肾脏病变有一定的保护作用,其机制可能是通过抑制氧化应激反应,下调糖尿病大鼠P22phoxmRNA的表达对2型糖尿痛模型大鼠肾脏产生保护作用。Objective： To investigate the effects of valsartan on renal lesions in type 2 diabetic rats. Method： Streptozotocin-indueed diabetic rats were randomly assigned to three groups ：control rats , type 2 diabetic rats and type 2 diabetic rats treated with Valsartan （10mg·kg^-1·d^-1） . After 6 weeks, content of MDA and activity of antioxidant enzymes including SOD in the renal tissue were compared among the groups. In addition, BG, Scr, BUN and urinary protein were measured respectively. P22phox gene expression was studied with a RT-PCR technique. Result： Compared with the untreated group, the level of BUN,Scr, renal MDA in Valsartan treated group were significantly decreased , and the activity of renal SOD were increased apparently. NADPH oxidatse subunit p22phox mRNA was significantly increased in the kidney of diabetic rats. Conclusion： Valsartan can amelibrate the renallesion in type 2 diabetic rats, antioxidation and inbibition of pzzphox-mRNA maybe the possible mechanism on nephro-protective action.

关 键 词：缬沙坦 2型糖尿病 NADPH氧化酶 糖尿病肾病 

分 类 号：R965.1[医药卫生—药理学]