HPLC测定小儿清肺止咳泡腾片中苦杏仁苷的研究  

Determination thd Content of Amygdalin in Xiaoer Qingfei Zhike Effervescent Tablet by HPLC

作　　者：常忆凌[1] 刘本[2] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100 [2]浙江大学宁波理工学院

出　　处：《中国药师》2007年第1期15-16,共2页China Pharmacist

基　　金：浙江省教育厅资助课题(20020702)

摘　　要：目的:建立用高效液相色谱法测定小儿清肺止咳泡腾片中苦杏仁苷含量的方法。方法:色谱柱为Diamonsil C_(18)柱(250 mm×4．6mm,5μm);流动相:甲醇-水(75:25);检测波长:218nm;流速1．0ml·min^(-1)。结果:苦杏仁苷在0．27～2．73μg的范围内成线性。r=0．9998,平均回收率为98．1%,RSD为2．36%。结论:该方法简便,可用于该药的质量控制。Objective： To establish a method of determination the content of amygdalin in Xiao＇er Qingfei Zhike effervescent tablet by HPLC. Method： Diamonsil C18 colum（ 150 mm ×4.6 mm, 5 μm） was used, mobile phase was methanol-water（75： 25）, determination wavelength was at 218 nm, and flow rate was 1.0 ml·min^-1. Result： The linear range of amygdalin was 0.27 -2.73 μg, r = 0.999 8, and an average recovery was 98.1%, RSD =2.36%. Conclusion： This method is simple and can be used to quality control of Xiaoer Qingfei Zhike effervescent tablet.

关 键 词：高效液相色谱法 苦杏仁苷 小儿清肺止咳泡腾片 

分 类 号：R927.2[医药卫生—药学]