内皮型一氧化氮合酶基因G894T多态性与心肌梗死的相关性  

The correlation between endothelial nitric oxide synthase gene G894T polymorphysim and myocardial infarction

作　　者：张爱元[1] 季祥武[1] 黄静[1] 管立学[1] 王敬先[1] 邵静波[1] 

机构地区：[1]潍坊市人民医院,山东省潍坊市261041

出　　处：《中国心血管病研究》2007年第1期10-12,共3页Chinese Journal of Cardiovascular Research

基　　金：山东省科学技术发展计划重点项目(2004GG2202040);吴阶平基金资助

摘　　要：目的探讨内皮型一氧化氮合酶(eNOS)基因G894T多态性与心肌梗死的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析,聚丙稀酰胺凝胶电泳方法检测确诊的225例冠心病患者和171名健康人的内皮型eNOS基因G894T多态性。结果①病例组与对照组比较GT+TT基因型频率较高(65/225和27/171),P<0.01,病例组T等位基因频率高于对照组(0.14和0.079)。②心肌梗死组GT+TT基因型频率高于非心肌梗死组(34/108和18/117),P<0.01,T等位基因频率高于非心肌梗死组(0.16和0.08),P<0.01。结论eNOS基因GT+TT基因型冠心病频率较高,并且易发生心肌梗死。Objective To investigate the distribution of the G894T polymorphysim at exon 7 in the endothelial nitric oxide synthase gene in Chinese Hart nationality and study the correlation between the G894T mutation at exon 7 in the endothelial nitric oxide synthase gene with myocardial infarction （MI）. Methods G894T polymorphysim at exon 7 in the endothelial nitric oxide synthase gene was measured in 225 patients with coronary heart disease （CHD） and 171 unrelated health individuals in control by a combination of polymerase chain reaction-restriction fragment length polymorphysm （PCR-RFLP） and direct AgNO3 staining technique. Results The frequencies of the GG＋GT genotypes were higher in patients with CHD（P〈0.01 ）. The GG＋GT genotypes were associated with the history of myocardial infarction （P〈0.01）.Conclusion The results indicated that the G894T polymorphysim at exon 7 in the endothelial nitric oxide ＇synthase gene in Chinese Han nationality was associated with coronary heart disease. The individual with GT or TT genotype is susceptible to myocardial infarction.

关 键 词：冠状动脉疾病 一氧化氮 基因 多态现象(遗传学) 

分 类 号：R541.4[医药卫生—心血管疾病]