白细胞介素-10基因多态性与乙型肝炎病毒感染的关系  

Genetic polymorphism of interleukin-10 in patients with hepatitis B infection

作　　者：严艳[1] 李卓[1] 李洪权[2] 李俊红[1] 刘英[2] 勾春燕[1] 高冀容[1] 单晶[1] 郭新会[1] 刘芳[1] 殷继明[1] 刘道洁[1] 谢贤春[1] 李辉[2] 

机构地区：[1]首都医科大学附属北京佑安医院肝炎研究所,北京市100069 [2]中国协和医科大学流行病教研室,北京市100009

出　　处：《世界华人消化杂志》2006年第36期3529-3533,共5页World Chinese Journal of Digestology

基　　金：Supported by the Momentous Science and Technology Project of Beijing Municipality;No.H020920020590

摘　　要：目的:探讨中国人白细胞介素-10基因启动子单核苷酸多态性与乙型肝炎病毒感染之后临床发展之间的关系.方法:分别采用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)和聚合酶链反应-序列特异性引物扩增法(PCR-SSP)检测478例慢性乙型肝炎患者,223例乙肝病毒携带者及267例自限性感染者基因组DNAIL-10基因启动子区域3个多态位点-592、-819、-1082的基因多态性,并进行相关性分析.结果:IL-10-1082A等位基因及AA基因型在慢性乙型肝炎患者组的频率明显高于自限性感染者组和乙肝病毒携带者组(A:χ2=37.72,P=0.000;χ2=45.23,P=0.000;AA:χ2=20.53,P=0.000;χ2=19.14,P=0.000).IL-10-819T等位基因及TT基因型在慢性乙型肝炎患者组的频率也高于自限性感染者组和乙肝病毒携带者组(T:χ2=10.5,P<0.001;χ2=17.38,P<0.001;TT:χ2=8.76,P=0.003;χ2=5.656,P=0.017).IL-10-592C/A等位基因频率和AA/CC/AC基因型在三组的分布没有统计学意义(P>0.05).结论:IL-10基因启动子多态性与乙肝病毒感染后临床发展过程可能相关.AIM： To investigate the correlation between the polymorphism of interleukin-10 （IL-10） gene promoters and the clinical phenotypes of hepatitis B virus （HBV） infection in Chinese population. METHODS： The genotypes of -819 locus in IL-10 promoter region were determined by polymerase chain reaction-sequence specific primer analysis （PCR-SSP）, and those of -1082 and -592 loci in IL-10 promoter region were detected by polymerase chain restriction fragment length polymorphism analysis （PCR-RFLP） in patients with chronic hepatitis B （CHB, n = 478）, selflimited HBV infection （SHI, n = 267） and chronic asymptomatic HBV carrier （AsC, n = 223）. RESULTS： The allele A and genotype AA frequencies of -1082 locus in IL-10 promoter region were significantly higher in CHB patients than those in SHI patients and AsC （A：X^ 2 = 37.72, P = 0.000; X^2 = 45.23, P = 0.000; AA： X^2 = 20.53, P = 0.000; X^2 = 19.14, P = 0.000）. The allele T and genotype Tr of -819 locus in IL-10 promoter region were also markedly higher in CHB patients than those in SHI patients and AsC （T： 2 = 10.5, P 〈 0.001; 2 = 17.38, P 〈 0.001; TT：X^2 = 8.76, P = 0.003; X^2 = 5.656, P = 0.017）. The polymorphism of -592 loci locus in IL-10 promoter region was not significantly different between the three groups （P 〉 0.05）. CONCLUSION： The genetic polymorphism of interleukin-10 may be associated with the clinical phenotypes of HBV infection.

关 键 词：白细胞介素-10 单核苷酸多态性 乙型肝炎病毒 

分 类 号：R512.62[医药卫生—内科学]